Ctbp1 (NM_001198860) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BARS; CtBP3/BARS; D4S115h; D5H4S115; D5H4S115E
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227657 representing NM_001198860 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGCGTCCGACCTCCCATCATGAACGGGCCCATGCACCCCCGGCCCCTGGTGGCGCTGCTGGATG GCCGGGACTGCACAGTGGAGATGCCTATCCTGAAGGATGTGGCCACAGTAGCCTTCTGTGATGCACAGTC CACACAGGAGATCCATGAGAAGGTACTGAATGAGGCTGTGGGTGCCCTGATGTACCATACCATCACACTG ACCAGAGAAGATCTGGAGAAGTTTAAAGCTCTTAGAATCATCGTCCGAATTGGCAGCGGGTTTGACAATA TCGACATCAAGTCAGCTGGGGATCTAGGCATCGCAGTGTGCAATGTGCCGGCAGCATCTGTGGAAGAAAC GGCAGACTCCACCCTGTGCCACATCCTGAACCTGTACCGACGAACCACCTGGCTACACCAGGCCCTTCGG GAAGGCACTCGAGTCCAGAGTGTAGAGCAGATCCGAGAGGTGGCTTCAGGAGCTGCCAGGATCCGTGGAG AGACCTTGGGCATCATTGGACTAGGTCGTGTGGGCCAGGCGGTGGCACTTCGGGCAAAGGCTTTTGGCTT CAACGTCCTCTTCTATGATCCATACCTATCTGATGGAATCGAGCGGGCCCTGGGGCTACAGCGCGTGAGC ACGCTGCAGGACCTGCTCTTCCACAGTGACTGCGTTACCCTGCATTGCGGCCTCAATGAGCACAACCACC ACCTCATCAATGACTTTACTGTCAAGCAGATGAGACAAGGAGCCTTTCTGGTGAACACAGCCCGTGGTGG CCTGGTGGATGAGAAGGCACTGGCCCAGGCCCTGAAGGAAGGGCGGATCCGTGGCGCAGCGCTGGACGTG CATGAGTCAGAGCCCTTCAGCTTTAGCCAGGGACCCTTAAAGGATGCACCCAACCTCATCTGCACACCCC ATGCTGCATGGTACAGTGAGCAGGCGTCCATTGAGATGAGAGAGGAGGCAGCCCGGGAAATCCGGCGAGC CATCACAGGCCGGATCCCAGATAGCTTGAAAAACTGTGTCAACAAGGACCACCTGACAGCCGCCACGCAC TGGGCCAGCATGGACCCTGCTGTGGTGCACCCTGAGCTCAATGGGGCTGCCTACAGGTACCCTCCAGGCG TCGTGAGTGTGGCCCCCACTGGCATCCCAGCTGCTGTGGAAGGGATTGTTCCCAGTGCCATGTCCCTGTC TCATGGCCTGCCCCCTGTGGCCCACCCACCCCACGCTCCCTCTCCTGGCCAGACTGTCAAGCCTGAGGCG GATAGAGACCATACGAGTGACCAGTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198860
Insert Size	1290 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198860.1, NP_001185789.1
RefSeq Size	2495 bp
RefSeq ORF	1290 bp
Locus ID	13016
UniProt ID	O88712
Gene Summary	Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate exon in the 5' region, initiates translation at an alternate start codon, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 3. It encodes a shorter isoform (2) with a distinct N-terminus compared to isoform 3.

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