Sept8 (NM_001252332) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW046166; Sepl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227656 representing NM_001252332 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCACCGACTTGGAACGCGTCTCGAACGCAGAGCCTGAGCCCCGGAGCCTGTCCCTGGGCGGCC ATGTTGGGTTTGACAGCCTCCCTGACCAGCTGGTCAGCAAGTCAGTCACTCAGGGCTTCAGCTTCAACAT ACTCTGTGTGGGCGAAACTGGGATTGGCAAGTCCACGCTAATGAACACACTCTTCAACACGACCTTTGAG ACTGAAGAAGCCAGTCACCATGAAGAGTGTGTGCGCCTGCGGCCTCAGACCTATGACCTCCAAGAGAGTA ACGTTCATCTCAAGCTGACCATCGTGGATGCTGTGGGCTTTGGCGATCAGATCAATAAGGATGACAGTTA CAGGCCCATAGTTGACTACATCGACGCGCAGTTTGAAAACTATCTGCAGGAGGAGTTGAAGATCCGCCGT TCCCTCTTTGACTATCACGACACGAGGATCCATGTTTGCCTCTACTTCATCACGCCCACCGGGCACTCCC TGAAGTCCCTGGATCTGGTGACCATGAAGAAACTAGATAGCAAGGTGAATATAATCCCCATCATTGCCAA GGCTGACACCATCTCCAAGAGCGAGCTCCACAAGTTCAAAATCAAGATCATGGGTGAGCTGGTCAGCAAC GGAGTCCAGATCTACCAGTTTCCCACCGATGACGAGGCTGTCGCCGAGATTAATGCAGTCATGAACGCAC ACCTGCCTTTTGCCGTGGTGGGCAGCACAGAGGAGGTGAAGGTGGGGAATAAGCTGGTTCGAGCACGACA GTACCCTTGGGGTGTGGTGCAGGTGGAGAATGAGAATCACTGTGACTTCGTGAAGCTGCGGGAGATGCTG ATCCGGGTGAACATGGAGGACCTGCGCGAGCAGACCCACAGCCGGCACTACGAGCTCTACCGCCGCTGCA AGTTGGAGGAGATGGGCTTCCAGGACAGTGACGGTGACAGCCAGCCCTTCAGCCTCCAAGAGACATATGA GGCAAAGAGGAAGGAGTTCCTAAGCGAGCTGCAGAGGAAGGAGGAAGAGATGCGACAGATGTTTGTCAAC AAAGTCAAGGAGACAGAGCTTGAGCTGAAGGAGAAGGAACGGGAGCTCCATGAGAAGTTTGAGCACCTGA AGCGAATCCACCAGGAGGAGAAGCGCAAGGTAGAGGAGAAGCGCAGGGAGCTGGAAGAGGAGACCAACGC CTTCAACTGCCGGAAGGCGGCCATGGAGGCCCTGCAGTCACAGGCCTTGCATGCCACTTCACAGCAGCCT CTGAGGAAAGACAAGGACAAGAAGAATTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001252332
Insert Size	1290 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252332.1, NP_001239261.1
RefSeq Size	4467 bp
RefSeq ORF	1290 bp
Locus ID	20362
UniProt ID	Q8CHH9
Gene Summary	Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (By similarity). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate 3' terminal exon, resulting in novel 3' coding region and 3' UTR, compared to variant 4. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 4.

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