Foxp3 (NM_001199348) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	JM2; scu; scurfin; sf
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227653 representing NM_001199348 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAACCCTAGGCCAGCCAAGCCTATGGCTCCTTCCTTGGCCCTTGGCCCATCCCCAGGAGTCTTGC CAAGCTGGAAGACTGCACCCAAGGGCTCAGAACTTCTAGGGACCAGGGGCTCTGGGGGACCCTTCCAAGG TCGGGACCTGCGAAGTGGGGCCCACACCTCTTCTTCCTTGAACCCCCTGCCACCATCCCAGCTGCAGCTG CCTACAGTGCCCCTAGTCATGGTGGCACCGTCTGGGGCCCGACTAGGTCCCTCACCCCACCTACAGGCCC TTCTCCAGGACAGACCACACTTCATGCATCAGCTCTCCACTGTGGATGCCCATGCCCAGACCCCTGTGCT CCAAGTGCGTCCACTGGACAACCCAGCCATGATCAGCCTCCCACCACCTTCTGCTGCCACTGGGGTCTTC TCCCTCAAGGCCCGGCCTGGCCTGCCACCTGGGATCAATGTGGCCAGTCTGGAATGGGTGTCCAGGGAGC CAGCTCTACTCTGCACCTTCCCACGCTCGGGTACACCCAGGAAAGACAGCAACCTTTTGGCTGCACCCCA AGGATCCTACCCACTGCTGGCAAATGGAGTCTGCAAGTGGCCTGGTTGTGAGAAGGTCTTCGAGGAGCCA GAAGAGTTTCTCAAGCACTGCCAAGCAGATCATCTCCTGGATGAGAAAGGCAAGGCCCAGTGCCTCCTCC AGAGAGAAGTGGTGCAGTCTCTGGAGCAGCAGCTGGAGCTGGAAAAGGAGAAGCTGGGAGCTATGCAGGC CCACCTGGCTGGGAAGATGGCGCTGGCCAAGGCTCCATCTGTGGCCTCAATGGACAAGAGCTCTTGCTGC ATCGTAGCCACCAGTACTCAGGGCAGTGTGCTCCCGGCCTGGTCTGCTCCTCGGGAGGCTCCAGACGGCG GCCTGTTTGCAGTGCGGAGGCACCTCTGGGGAAGCCATGGCAATAGTTCCTTCCCAGAGTTCTTCCACAA CATGGACTACTTCAAGTACCACAATATGCGACCCCCTTTCACCTATGCCACCCTTATCCGATGGGCCATC CTGGAAGCCCCGGAGAGGCAGAGGACACTCAATGAAATCTACCATTGGTTTACTCGCATGTTCGCCTACT TCAGAAACCACCCCGCCACCTGGAAGAATGCCATCCGCCACAACCTGAGCCTGCACAAGTGCTTTGTGCG AGTGGAGAGCGAGAAGGGAGCAGTGTGGACCGTAGATGAATTTGAGTTTCGCAAGAAGAGGAGCCAACGC CCCAACAAGTGCTCCAATCCCTGCCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199348
Insert Size	1290 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199348.1, NP_001186277.1
RefSeq Size	3690 bp
RefSeq ORF	1290 bp
Locus ID	20371
UniProt ID	Q99JB6
Gene Summary	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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