Syt6 (NM_001276679) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110037A08Rik; AW048930; sytVI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227632 representing NM_001276679 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGAGGAAGAAAGAGGCCTCCAGCCCTTCTTCTGCTAACCCTGCTTCAGAGACTCTCCAGAGCC CCAGCTCCAGAGGCAACATGGCGGATAAGCTGAAGGACCCCAGTGCCCTGGGCTTCCTGGAGGCAGCTGT GAAGATCAGCCACACTTCCCCAGACATCCCAGCCGAGGTGCAGATGTCGGTCAAAGAGCACATCATGCGC CACACAAAGCTGCAGCGACAGACCACAGAGCCAGCATCCTCCACCAGGCACACATCCTTCAAGCGCCACC TGCCACGACAGATGCATGTCTCCAGCGTGGACTATGGCAATGAGCTGCCGCCGGCTGCCGCCGAGCAGCC CACCAGTATTGGCCGTATCAAGCCTGAGCTTTACAAGCAGAAGTCAGTGGATGGGGATGATGCCAAGTCG GAGGCCGCCAAGAGCTGTGGGAAGATCAACTTCAGCCTCCGCTATGACTATGAAAGCGAGACGCTGATTG TGCGCATCCTGAAGGCCTTTGACCTCCCTGCCAAGGACTTTTGCGGAAGTTCTGACCCTTATGTTAAGAT CTACCTCCTGCCTGACCGCAAGTGCAAGCTGCAGACCCGGGTGCACCGCAAGACCCTGAACCCCACCTTT GATGAGAACTTCCACTTCCCCGTGCCCTACGAGGAGCTGGCTGACCGCAAGCTGCATCTCAGTGTCTTTG ACTTTGACCGCTTCTCCCGCCATGACATGATTGGGGAGGTCATCCTGGACAATCTCTTTGAGGCCTCTGA CCTGTCCCGGGAGACCTCCATCTGGAAGGACATCCAGTACGCTACTAGTGAAAGTGTGGACTTGGGAGAG ATCATGTTCTCCCTCTGCTACCTGCCCACAGCAGGCAGGCTCACCCTCACAGTGATCAAGTGTCGAAATC TCAAAGCAATGGACATCACAGGCTACTCAGATCCCTACGTCAAAGTATCCCTACTGTGTGATGGACGGAG GCTGAAAAAGAAGAAAACGACCATAAAAAAGAACACCCTAAACCCCATCTACAACGAGGCCATCATCTTT GACATCCCCCCAGAAAACATGGACCAAGTGAGCCTGCTCATCTCCGTTATGGACTATGATAGAGTTGGCC ACAACGAGATCATAGGAGTCTGTAGAGTGGGGATCAATGCTGAAGGCCTTGGCAGGGACCACTGGAATGA GATGTTGGCGTACCCACGAAAGCCCATTGCGCACTGGCACTCCTTGGTGGAGGTAAAGAAATCCTTCAAA GAGGGGACCCCTCGGTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276679
Insert Size	1281 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276679.1, NP_001263608.1
RefSeq Size	1599 bp
RefSeq ORF	1281 bp
Locus ID	54524
Gene Summary	May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm (PubMed:15774481).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3, 4 and 6 encode the same isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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