Pacsin3 (NM_001289678) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921507A02Rik; 6330413E15Rik; AW413130
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227628 representing NM_001289678 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCAGAGGAGGACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGGAGGCTGGCAACTACAGAC GGACGGTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGGACCTGGTTAGCTGCTTCCAAGAACGTGC CCGCATTGAGAAGGCCTACGCCCAGCAGCTGGCTGACTGGGCCCGCAAGTGGAGGGGTGCTGTGGAGAAG GGCCCACAGTACGGCACCCTGGAGAAGGCCTGGCATGCCTTCTTCACTGCGGCTGAGAGGCTGAGCGAGC TGCACTTGGAGGTGAGGGAGAAGTTGCACGGTCCAGACAGCGAGCGAGTGCGGACCTGGCAGCGAGGGGC TTTCCACCGGCCAGTGCTGGGGGGCTTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTCCGTAAAGCTCAG AAGCCCTGGCTAAAGAGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACCACACAGCCCGCAAGGATG AGAAGACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACAGCTCCATGTCCCAGGAGCAGCTTCGAAA ATTACAGGAGCGGGTGGGCCGCTGCACCAAGGAGGCAGAGAAGATGAAAACCCAGTATGAGCAGACCCTG GCCGAGCTAAATCGCTACACCCCACGCTACATGGAGGACATGGAGCAGGCTTTTGAGAGCTGCCAGGCTG CTGAGCGCCAGCGGCTTCTCTTCTTCAAGGATGTCTTGCTCACCCTGCATCAACACCTCGACCTCTCCAG TAGCGACAAGTTCCATGAACTCCATCGAGACCTGCAGCAGAGCATTGAGGCTGCCAGTGATGAAGAGGAT CTGCGCTGGTGGCGGAGCACACACGGGCCTGGCATGGCCATGAACTGGCCACAGTTTGAGGAATGGTCGT TAGACACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCCGGAGCCCAGATGAGGTTACTCTGACCAG CATTGTACCGACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCATCGTCCCCGGGTAGCGGGCAGGAC GAGGACTGGTCAGATGAGGAGAGTCCCCGGAAAGTTGCCACTGGGGTGAGGGTACGGGCGCTTTATGACT ATGCTGGCCAGGAGGCTGATGAGCTGAGCTTCCGAGCAGGGGAAGAGTTGCTGAAGATGAGCGAGGAGGA TGAGCAGGGCTGGTGCCAGGGCCAGCTGCAGAGTGGCCGCATTGGTCTGTACCCTGCCAATTACGTAGAA TGTGTGGGCGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289678
Insert Size	1275 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289678.1, NP_001276607.1
RefSeq Size	1864 bp
RefSeq ORF	1275 bp
Locus ID	80708
UniProt ID	Q99JB8
Gene Summary	Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 through 4 encode the same protein.

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