Runx2 (NM_001271631) Mouse Untagged Clone

CAT#: MC227617

Runx2 (untagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 6



  "NM_001271631" in other vectors (1)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AM; AML3; Cbf; Cbfa; Cbfa-1; Cbfa1; LS3; Os; Osf2; PEB; Pebp2a1; PEBP2aA; Pebpa2a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227617 representing NM_001271631
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTATTCCTGTAGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCA
AGATGAGCGACGTGAGCCCGGTGGTGGCTGCGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCA
GCAGCAACAACAGCAACAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGGAGGCGGCCGCAGCAGCAGCG
GCGGCAGCGGCGGCGGCAGCAGCGGCGGCGGCCGCAGTGCCCCGATTGAGGCCGCCGCACGACAACCGCA
CCATGGTGGAGATCATCGCGGACCACCCGGCCGAACTGGTCCGCACCGACAGTCCCAACTTCCTGTGCTC
CGTGCTGCCCTCGCACTGGCGGTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTTGTAGCCCTCGGAGAG
GTACCAGATGGGACTGTGGTTACCGTCATGGCCGGGAATGATGAGAACTACTCCGCCGAGCTCCGAAATG
CCTCCGCTGTTATGAAAAACCAAGTAGCCAGGTTCAACGATCTGAGATTTGTGGGCCGGAGCGGACGAGA
TCCCAGGCAGGCACAGTCTTCCCCACCGTGGTCCTATGACCAGTCTTACCCCTCCTATCTGAGCCAGATG
ACATCCCCATCCATCCACTCCACCACGCCGCTGTCTTCCACACGGGGCACCGGGCTACCTGCCATCACTG
ACGTGCCCAGGCGTATTTCAGATGATGACACTGCCACCTCTGACTTCTGCCTCTGGCCTTCCTCTCTCAG
TAAGAAGAGCCAGGCAGGTGCTTCAGAACTGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCA
TCCCTCACTGAGAGCCGCTTCTCCAACCCACGAATGCACTACCCAGCCACCTTTACCTACACCCCGCCAG
TCACGTCAGGCATGTCCCTCGGCATGTCCGCCACCACTCACTACCACACGTACCTGCCACCACCCTACCC
CGGCTCTTCCCAAAGCCAGAGTGGACCCTTCCAGACCAGCAGCACTCCATATCTCTACTATGGTACTTCG
TCAGCATCCTATCAGTTCCCAATGGTACCCGGGGGAGACCGGTCTCCTTCCAGGATGGTCCCACCATGCA
CCACCACCTCGAATGGCAGCACGCTATTAAATCCAAATTTGCCTAACCAGAATGATGGTGTTGACGCTGA
CGGAAGCCACAGCAGTTCCCCAACTGTTTTGAATTCTAGCGGCAGAATGGATGAGTCTGTTTGGCGGCCA
TATTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001271631
Insert Size 1266 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001271631.1, NP_001258560.1
RefSeq Size 5724 bp
RefSeq ORF 1266 bp
Locus ID 12393
Gene Summary This gene encodes a member of the runt domain-containing family of transcription factors. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Sep 2015]
Transcript Variant: This variant (6) represents use of a proximal promoter. It lacks two alternate exons including the 5' UTR and a portion of the 5' coding region, initiates translation in an alternate 5' segment, and lacks two internal in-frame coding exons, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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