Sh2b2 (NM_001302939) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Aps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227608 representing NM_001302939 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGTGCCACCCCCAGCTCGGCCGCCGCCCCCGCCCCAGTCCCCGACTGGCGGCAATTCTGCGAGC TGCACGCGCAGGTGGCTGCTGTGGACTTCGCACACAAGTTCTGCCGCTTCCTTCGGGACAACCCGACCTA CGACACGCCTGACGCTGGGACCTCATTCTCCCGCCACTTTGCAGCCAACTTCTTGGCTGTCTTCAGTGAG GAGGTGCGCCGCGTGCTGGGGTCAGCAGCCGACACTATGGAACCCGAGCCTGCGGTGACCTCGGTGACAT CTGCGCTCAAGACTGCAACCTATGGCCACTCCCGCAGCTCCGAGGATGTGTCTGCCCACGCGGCTACCAA GGCGCGTGTCCGAAAGGGATTCTCGCTGCGTAACATGAGCCTGTGTGTGGTGGATGGAGTGCGCGACCTG TGGCACCGGCGCTCGTCCCCAGAGCCCGATGGTGGGGCCACCCCCAAGGCCGCCGAGCCTGCGTCGGAGC CTCGTGACAAATGGACGCGACGCCTGCGGCTAGCACGAACCCTGGCAGCCAAAGTGGAGTTAGTGGACAT CCAGCGTGAGGGCGCCCTGCGCTTCATGGTGGCAGACGACGCGGCCTCAGGGCCTGGAGGCACTGCGCAG TGGCAGAAGTGTCGCCTGCTCCTGCGGAGGGCTGTGGCTGGGGAACGTTTCCGCCTGGAGTTCTTCGTGC CACCCAAGGCTTCCAGACCCAAGGTCAGCATCCCCCTGTCGGCCATCATTGAGGTCCGAACCACTATGCC CCTGGAAATGCCTGAAAAGGACAACACATTTGTGCTCAAGGTGGAGAACGGAGCAGAATACATCCTGGAA ACGATAGACTCACTGCAGAAGCACTCGTGGGTGGCTGACATCCAGGGCTGTGTGGATCCTGGGGACAGTG AGGAAGACACAGGACTGTCTTGTGCACGGGGAGGCTGCCTGGCCAGCCGCGTGGCCTCCTGCAGCTGTGA GCTCCTGACAGATGCAGACATGCCCCGGCCCCCCGAGACGACGACAGCGGTGGGTGCTGTGGTCACAGCT CCACATGGCCGAGCTCGTGACACCGTGGGAGAGTCCCTGGCCCACGTTCCCCTGGAGACTTTCCTGCAAA CCCTGGAGTCTTCAGGTGGTGTCAGTGAGAACAATAACCCAGGTACCACTGGGGTCCCCACCCCCTTCTA CAGGGACCCATGTGGAGCCTCAGAAGTGGTGACATTTCAGCCAGTTGTGTCTACAGTGGTTTTGGCTTTC TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302939
Insert Size	1263 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302939.1, NP_001289868.1
RefSeq Size	1894 bp
RefSeq ORF	1263 bp
Locus ID	23921
UniProt ID	Q9JID9
Gene Summary	Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. May be involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. May induce cytoskeletal reorganization via interaction with VAV3 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.

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