Nxf1 (NM_001276704) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Mex67; Mvb1; Tap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227594 representing NM_001276704 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAAGGGAAGTCATACAACGAGCATGATGACCGTGTTAGTTTCCCTCAAAGAAGAAAGAAAG GCCGGGGGCCTTTCCGATGGAAGTGTGGTGAGGGGAATCGTAGGTCTGGAAGAGGTGGTTCTGGTGTACA GTCTTCCCGATTTGAGGAAGATGATGGCGATGTAGCAATGAATGATCCCCAGGATGGCCCCCGAGTAAGA TACAATCCCTATACCAACCGGCCTAATCGTCGGGGAGATGGTTGGCATGATCGAGATCGAATTCATATTA CTGTACGGAGAGACAGAGCTCCTGCAGAGAGAGGAGGGGCTGGCACCAGTCAAGATGGGACCACCAAGAA CTGGTTCAAGATTACAATTCCTTATGGTAGAAAGTATGACAAAACGTGGCTTCTGAGCATGATTCAGAGC AAATGCAGTGTCCCCTTCAATCCCATCGAGTTTCACTATGAAAATACACGGGCCCATTTTTTTGTGGAGG ATGCCACTACTGCTTCTGCATTAAAGGGTGTCAACCATAAGATCCAGGATCGAGAAAACAGAAGGATATC TATCATCATCAATGCTTCTGCTCCACCCTACACTGTACAGAATGAACTGAAGCCCGAACAAATAGAGCAG CTCAAGCTGATCATGAGCAAACGATATGATGGCAACCAACAAGCACTTGACCTCAAGGGGCTCCGCTCAG ACCCAGATTTGGTGGCCCAGAACATCGATGTTGTTCTAAACCGCAGAAGCTGTATGGCAGCCACCCTGCG GATCATTGAAGAGAACATTCCTGAGCTATTGTCTTTGAACTTGAGCAGCAACAGGCTATACAAGCTGGAT GATATGTCTAGCATTGTGCAGAAGGCGCCAAACCTAAAGACCCTAAATCTCTCCGGAAACGAATTGAAGA CTGAGCGGGAATTAGACAAGATAAAAGGGCTGAAGCTGGAAGAGCTGTGGCTTGACAGAAACCCCATGTG TGACAACTTCGGAGACCAGTCCAGCTATATCAGGTCAGTTGTAGCCTCTGTCTCCCCTCCTGGGGACATT CACCCCTGGGAGGCTGAGCTCATGCACCCTGACCAGTGCCCGTCTACAGGAGAACATGCAGCCCTGAGCT GGAACCACCTGTTCTTTGGGAGGAGGATCATGTATTCCTTTCTGCCTGTCTGTTGTCTCCTAGCTCTGTC CCAGATCTCTTCTTTCTTCTCACCTGACTTCTGTTCTCTAGTTCTGGCCTTTCTCCTTCTTTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276704
Insert Size	1257 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276704.1, NP_001263633.1
RefSeq Size	4035 bp
RefSeq ORF	1257 bp
Locus ID	53319
Gene Summary	Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate internal segment and transcription extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.

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