Cept1 (NM_001293694) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9930118K05Rik; mCEPT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227584 representing NM_001293694 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGGCATCGGTCAACAAGGAAACGATGTGGAGATTCTCACCCAGAGTCCCCTGTGGGCTTTGGGC ATATGAGTACTACAGGGTGTGTATTAAATAAATTGTTTCAGTTACCGACACCACCACTGTCAAGACACCA GTTAAAACGGCTAGAAGAACACAGGTATCAGAGTGCTGGACGGTCCCTTCTCGAGCCCTTAATGCAAGGA TACTGGGAATGGCTAGTTGGAAGAGTACCCTCATGGATTGCCCCAAATCTCATCACCATCATTGGACTAT CAATAAATATCTGTACAACTATTTTGTTAGTCTTCTACTGCCCTACAGCTACAGAGCAGGCACCTCTGTG GGCTTATATTGCCTGTGCTTGTGGCCTTTTCATTTACCAGTCTCTGGATGCCATAGATGGGAAACAGGCC AGAAGAACTAACAGTAGCTCTCCTCTGGGAGAACTTTTTGATCATGGCTGTGACTCACTATCGACAGTTT TTGTGGTTCTTGGAACTTGTATTGCAGTACAACTTGGGACAAATCCTGACTGGATGTTTTTTTGTTGTTT TGCTGGGACATTCATGTTCTATTGTGCACACTGGCAAACATATGTTTCTGGAACATTGCGATTTGGAATA TTCGATGTTACAGAGTCCCAGATCCTAATTATATTATTCCAGCTTCTCTCAGGAACCGTGGGGCCTTGGT TCTGGAACTTCACGATTCCTGTGTTGAATATTCAAATGAAACTTCTTCCTGCACTTTGTACTGTGGCAGG GACCATATTTTCCTGTACAAATTACTTCCGTGTAATCTTCACAGGTGGTGTTGGCAAAAATGGGTCAACC ATAGCAGGAACAAGTGTCCTTTCTCCTTTTCTGCATATTGGATCAGTGATCACGTTAGCTGTTATGATCT ACAAGAAATCAGCAGTTCAGCTTTTTGAAAAGCATCCATGTCTTTATATACTGACATTTGGTTTTGTGTC GGCTAAAATCACTAATAAGCTTGTGGTTGCTCACATGACGAAAAGTGAAATGCATTTGCATGACACAGCA TTCATAGGTCCAGCACTTCTGTTTCTGGACCAGTATTTTAACAGCTTCATTGATGAATATATAGTACTTT GGATTGCCTTGATCTTCTCTTTCTTTGATTTGATTCGTTACTGTGTCAGTGTTTGCAATCAGATTGCATC TCACCTGCATATACATGTCTTCAGAATTAAAGCCTCTACAGCTCACTCTAATCATCACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293694
Insert Size	1251 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293694.1, NP_001280623.1
RefSeq Size	2814 bp
RefSeq ORF	1251 bp
Locus ID	99712
UniProt ID	Q8BGS7
Gene Summary	Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon and contains an alternate exon but maintains the reading frame compared to variant 1. The encoded isoform (4) is the same length as isoform 1, but contains a distinct internal segment. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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