Mknk1 (NM_001285488) Mouse Untagged Clone

CAT#: MC227577

Mknk1 (untagged) - Mouse MAP kinase-interacting serine/threonine kinase 1 (Mknk1), transcript variant 2



  "NM_001285488" in other vectors (1)

CNY 3,900.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410048M24Rik; Mnk; Mnk1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227577 representing NM_001285488
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGCAGTGAGCCCCTTCCCATCGTGGATTCTGACAAGAGGAGGAAGAAGAAGCGTAAGACCCGGG
CCACCGACTCTCTGCCAGGAAAGTTTGAAGATGTGTACCAGCTGACCTCGGAATTGCTGGGAGAAGGAGC
CTATGCCAAAGTCCAGGGTGCTGTGAACCTACAGAGTGGGAAGGAGTATGCTGTCAAAATCATCGAGAAG
CAAGCCGGGCACAGTCGAAGTCGAGTGTTCCGTGAGGTGGAGACACTGTATCAGTGTCAAGGGAACAGGA
ACATTTTGGAGCTGATTGAATTCTTTGAAGATGACACACGGTTTTACTTGGTCTTTGAGAAATTGCAAGG
AGGCTCCATCCTAGCACACATCCAGAAGCGGAAGCACTTCAATGAGCGAGAAGCCAGTAGAGTGGTGCGG
GACGTCGCCACTGCCCTTGACTTCCTGCACACTAAAGGCATTGCTCACCGTGATCTGAAGCCAGAAAACA
TACTGTGTGAATCTCCAGAAAAGGTGTCTCCGGTGAAAATTTGTGACTTTGACTTGGGCAGTGGGGTAAA
GTTGAACAACTCCTGCACTCCCATAACCACGCCAGAGCTGACTACTCCATGCGGCTCTGCAGAGTATATG
GCACCTGAGGTGGTGGAGGTCTTTAGGGACGAGGCTACTTTCTATGACAAGCGCTGTGACCTGTGGAGCC
TGGGTGTGGTCCTCTACATCATGCTGAGTGGCTACCCCCCCTTTGTAGGTCACTGTGGGGCTGACTGTGG
CTGGGACCGGGGAGAGGTATGCAGGATGTGCCAGAACAAGCTGTTTGAGAGCATCCAGGAAGGCAAATAC
GAGTTTCCTGACAAAGACTGGGCTCACATCTCCAACGAGGCCAAAGACCTCATCTCTAAGCTCCTGGTTC
GAGATGCGAAGCAAAGACTCAGTGCTGCCCAGGTTCTGCAGCATCCATGGGTACAAGGGCAAGCTCCAGA
AAGGGGACTCCCCACGCCGCAAGTCCTTCAGAGAAACAGCAGCACCATGGACTTGACTCTCTTCGCAGCT
GAGGCCATTGCCCTGAACCGCCAGCTGTCTCAGCATGAGGAGAATGAACTGGCCGAGGAACAGGAGGCCC
TAGCTGAGGGCCTCTGCTCCATGAAGCTGTCCCCTCCATCCAAATCTCGCCTGGCTCGAAGGCGAGCCCT
GGCCCAGGCTGGCCGAAGCCGAGATGCAAACCCATGTTTGACACCGGCAGGGCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001285488
Insert Size 1248 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001285488.1, NP_001272417.1
RefSeq Size 2598 bp
RefSeq ORF 1248 bp
Locus ID 17346
UniProt ID O08605
Gene Summary This gene encodes a serine-threonine protein kinase that is activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases, and it may function in cytokine and environmental stress responses. This kinase is required for phosphorylation of eukaryotic translation initiation factor 4E but it is not required for cell growth during development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Oct 2013]
Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Both variants 2 and 3 encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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