Mknk1 (NM_001285488) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410048M24Rik; Mnk; Mnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227577 representing NM_001285488 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCAGTGAGCCCCTTCCCATCGTGGATTCTGACAAGAGGAGGAAGAAGAAGCGTAAGACCCGGG CCACCGACTCTCTGCCAGGAAAGTTTGAAGATGTGTACCAGCTGACCTCGGAATTGCTGGGAGAAGGAGC CTATGCCAAAGTCCAGGGTGCTGTGAACCTACAGAGTGGGAAGGAGTATGCTGTCAAAATCATCGAGAAG CAAGCCGGGCACAGTCGAAGTCGAGTGTTCCGTGAGGTGGAGACACTGTATCAGTGTCAAGGGAACAGGA ACATTTTGGAGCTGATTGAATTCTTTGAAGATGACACACGGTTTTACTTGGTCTTTGAGAAATTGCAAGG AGGCTCCATCCTAGCACACATCCAGAAGCGGAAGCACTTCAATGAGCGAGAAGCCAGTAGAGTGGTGCGG GACGTCGCCACTGCCCTTGACTTCCTGCACACTAAAGGCATTGCTCACCGTGATCTGAAGCCAGAAAACA TACTGTGTGAATCTCCAGAAAAGGTGTCTCCGGTGAAAATTTGTGACTTTGACTTGGGCAGTGGGGTAAA GTTGAACAACTCCTGCACTCCCATAACCACGCCAGAGCTGACTACTCCATGCGGCTCTGCAGAGTATATG GCACCTGAGGTGGTGGAGGTCTTTAGGGACGAGGCTACTTTCTATGACAAGCGCTGTGACCTGTGGAGCC TGGGTGTGGTCCTCTACATCATGCTGAGTGGCTACCCCCCCTTTGTAGGTCACTGTGGGGCTGACTGTGG CTGGGACCGGGGAGAGGTATGCAGGATGTGCCAGAACAAGCTGTTTGAGAGCATCCAGGAAGGCAAATAC GAGTTTCCTGACAAAGACTGGGCTCACATCTCCAACGAGGCCAAAGACCTCATCTCTAAGCTCCTGGTTC GAGATGCGAAGCAAAGACTCAGTGCTGCCCAGGTTCTGCAGCATCCATGGGTACAAGGGCAAGCTCCAGA AAGGGGACTCCCCACGCCGCAAGTCCTTCAGAGAAACAGCAGCACCATGGACTTGACTCTCTTCGCAGCT GAGGCCATTGCCCTGAACCGCCAGCTGTCTCAGCATGAGGAGAATGAACTGGCCGAGGAACAGGAGGCCC TAGCTGAGGGCCTCTGCTCCATGAAGCTGTCCCCTCCATCCAAATCTCGCCTGGCTCGAAGGCGAGCCCT GGCCCAGGCTGGCCGAAGCCGAGATGCAAACCCATGTTTGACACCGGCAGGGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285488
Insert Size	1248 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285488.1, NP_001272417.1
RefSeq Size	2598 bp
RefSeq ORF	1248 bp
Locus ID	17346
UniProt ID	O08605
Gene Summary	This gene encodes a serine-threonine protein kinase that is activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases, and it may function in cytokine and environmental stress responses. This kinase is required for phosphorylation of eukaryotic translation initiation factor 4E but it is not required for cell growth during development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Both variants 2 and 3 encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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