Kcnk2 (NM_001281847) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430027H14Rik; AI848635; TREK-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227571 representing NM_001281847 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCAGTGGCGGCCCCTGACTTGCTGGATCCCAAGTCTGCTGCTCAGAACTCCAAACCGAGGCTCT CATTCTCCTCAAAACCCACCGTGCTTGCTTCCCGGGTGGAGAGTGACTCGGCCATTAATGTTATGAAATG GAAGACAGTCTCCACGATTTTCCTGGTGGTCGTCCTCTACCTGATCATCGGAGCCACGGTGTTCAAGGCA TTGGAGCAGCCTCAGGAGATTTCCCAGAGGACCACCATTGTGATCCAGAAGCAGACCTTCATAGCCCAGC ATGCCTGCGTCAACTCCACCGAGCTGGACGAACTCATCCAGCAAATAGTGGCAGCAATAAACGCAGGGAT TATCCCCTTAGGAAACAGCTCCAATCAAGTTAGTCACTGGGACCTCGGAAGCTCTTTCTTCTTTGCTGGT ACTGTTATCACAACCATAGGATTTGGAAACATCTCCCCACGAACTGAAGGTGGAAAAATATTCTGCATCA TCTATGCCTTGCTGGGAATTCCCCTCTTTGGCTTTCTACTGGCTGGGGTTGGTGATCAGCTAGGAACTAT ATTTGGAAAAGGAATTGCCAAAGTGGAAGACACATTTATTAAGTGGAATGTTAGTCAGACGAAGATTCGT ATCATCTCCACCATCATCTTCATCCTGTTTGGCTGTGTCCTCTTTGTGGCTCTCCCTGCGGTCATATTCA AGCACATAGAAGGCTGGAGCGCCCTGGACGCTATCTATTTTGTGGTTATCACTCTGACGACCATTGGATT TGGAGACTACGTGGCAGGTGGATCAGACATTGAATATCTGGACTTCTACAAGCCTGTGGTGTGGTTCTGG ATCCTCGTTGGGCTGGCCTACTTTGCAGCTGTTCTGAGCATGATTGGGGACTGGCTACGGGTGATCTCTA AGAAGACGAAGGAAGAGGTGGGAGAGTTCAGAGCGCATGCCGCTGAGTGGACAGCCAATGTCACGGCCGA GTTCAAGGAAACGAGGAGGCGGCTGAGCGTGGAGATCTACGACAAGTTCCAGCGTGCCACATCCGTGAAG CGGAAGCTCTCCGCAGAGCTGGCGGGCAACCACAACCAGGAACTGACTCCGTGTAGGAGGACCCTGTCTG TGAACCACCTGACCAGCGAGAGGGAAGTCCTGCCTCCCTTGCTGAAGGCTGAGAGCATCTATCTGAACGG TCTGACACCACACTGTGCTGGTGAGGACATAGCTGTCATTGAGAACATGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281847
Insert Size	1245 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281847.1, NP_001268776.1
RefSeq Size	3314 bp
RefSeq ORF	1245 bp
Locus ID	16526
Gene Summary	Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate 5' terminal exon and thus differs in the 5' UTR and 5' coding region compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1.

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