Zdhhc6 (NM_025883) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2400007G07Rik; 5930409M18Rik; AI462345; C77369
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227565 representing NM_025883 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATATTCTGCTCAGTAATCAAGTTTGAAAATCTTCAAGACTTAAGGAGACTGTGTCATTGGGGTC CCATCATAGCCCTGGGTGTTATAGCAATATGTTCCACAATGGCCATGATCGACTCTGTCTTGTGGTATTG GCCCTTACATACAACTGGAGGAAGTGTGAACTTCATCATGTTGATAAATTGGACCGTCATGATTCTTTAT AATTACTTCAATGCCATGTTTGCTGGTCCTGGCTTCGTCCCTCGGGGGTGGAAACCGGAAAAGTCTCAGG ATAGCATGTATCTCCAGTATTGTAAAGTCTGCCAAGCATACAAGGCGCCACGTTCACACCATTGCAGAAA GTGTAACAGATGTGTAATGAAGATGGACCACCACTGCCCCTGGATCAACAACTGCTGTGGTCACCAGAAC CACGCCTCGTTCACGCTCTTCCTCCTGTTGGCCCCTCTGGGCTGCACTCATGCTGCTTTCATCTTCGTGA TGACAATGTACACACAGCTTTATAATCGGCTCTCCTTTGGGTGGAACACAGTCAAGATTGACATGAGTGC AGCCAGGAGAGATCCACCTCCAATTGTTCCCTTCGGATTGGCTGCGTTTGCCGCCACCTTGTTTGCCTTG GGATTGGCTTTGGGAACAACCATAGCTGTTGGGATGTTGTTTTTCATACAGATAAAAATTATTCTCAGAA ACAAAACTTCTATTGAATCATGGATTGAAGAAAAGGCTAAAGATCGCATTCAGTATTACCAACTGGATGA AGTCTTCATTTTTCCTTATGATATGGGAAGTAAGTGGAAGAACTTTAAACAGGTGTTTACATGGTCGGGA GTTCCTGAAGGAGATGGACTTGAGTGGCCAATACGAGAGGGCTGTGACCAGTACAGCCTCACAATAGAAC AGTTGAAGCAAAAGGCAGACAAGAGAGTCAGAAGTGTTCGCTATAAAGTCATAGAAGATTACAATGGTGC CTGCTGCCCTTTGAATAGAGGAGTGAGAACCTTCTTTACGAGCCCCTGCACTGAGGAGCCTCGGATCCGG CTGCAGAAAGGAGAGTTCATCTTAGCAACGAGAGGGCTGCGATACTGGTTGTATGGAGACAAAATTCTTG ATGATTCCTTCATAGAAGGTACTTCAAGAGTAAGAGGGTGGTTTCCTAGAAACTGTGTGGAAAAGTGTCC CTGTGATGGTGACTCAGATCCAGCCCCAGAGGGGGAGAAGAAGAACAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_025883
Insert Size	1242 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_025883.3, NP_080159.3
RefSeq Size	2182 bp
RefSeq ORF	1242 bp
Locus ID	66980
UniProt ID	Q9CPV7
Gene Summary	Endoplasmic reticulum palmitoyl acyltransferase that mediates palmitoylation of proteins such as AMFR, CALX, ITPR1 and TFRC (PubMed:25368151). Palmitoylates calnexin (CALX), which is required for its association with the ribosome-translocon complex and efficient folding of glycosylated proteins (By similarity). Mediates palmitoylation of AMFR, promoting AMFR distribution to the peripheral endoplasmic reticulum (By similarity). Together with SELENOK, palmitoylates ITPR1 in immune cells, leading to regulate ITPR1 stability and function (PubMed:25368151).[UniProtKB/Swiss-Prot Function]

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