Bmp8a (NM_001256019) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Bmp7; Bmp7r1; O; OP-2; OP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227560 representing NM_001256019 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGCGTCCCGGGCCACTCTGGCTATTGGGCCTTGCTCTGTGCGCGCTGGGAGGCGGCCACGGTC CGCGTCCCCCGCACACCTGTCCCCAGCGTCGCCTGGGAGCGCGCGAGCGCCGCGACATGCAGCGTGAAAT CCTGGCGGTGCTCGGGCTACCGGGACGGCCCCGACCCCGTGCACAACCCGCCGCTGCCCGGCAGCCAGCG TCCGCGCCCCTCTTCATGTTGGACCTATACCACGCCATGACCGATGACGACGACGGCGGGCCACCACAGG CTCACTTAGGCCGTGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAACGCGACCGTACCCTGGGCTA CCAGGAGCCACACTGGAAGGAATTCCACTTTGACCTAACCCAGATCCCTGCTGGGGAGGCTGTCACAGCT GCTGAGTTCCGGATCTACAAAGAACCCAGCACCCACCCGCTCAACACAACCCTCCACATCAGCATGTTCG AAGTGGTCCAAGAGCACTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCTCCGATCTGG GGACGAGGGCTGGCTGGTGCTGGACATCACAGCAGCCAGTGACCGATGGCTGCTGAACCATCACAAGGAC CTGGGACTCCGCCTCTATGTGGAAACCGCGGATGGGCACAGCATGGATCCTGGCCTGGCTGGTCTGCTTG GACGACAAGCACCACGCTCCAGACAGCCTTTCATGGTAACCTTCTTCAGGGCCAGCCAGAGTCCTGTGCG GGCCCCTCGGGCAGCGAGACCACTGAAGAGGAGGCAGCCAAAGAAAACGAACGAGCTTCCGCACCCCAAC AAACTCCCAGGGATCTTTGATGATGGCCACGGTTCCCGCGGCAGAGAGGTTTGCCGCAGGCATGAGCTCT ACGTCAGCTTCCGTGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAGGGCTACTCTGCCTATTACTG TGAGGGGGAGTGTGCTTTCCCACTGGACTCCTGTATGAACGCCACCAACCATGCCATCTTGCAGTCTCTG GTCAGTACCACAGTAGCCTGCTGCGACAGGTGGAGCGGGGTGCACCTGATGAAGCCAGATGTTGTCCCCA AGGCATGCTGTGCACCCACCAAACTGAGTGCCACCTCTGTGCTGTACTATGACAGCAGCAACAATGTCAT CCTGCGTAAACACCGTAACATGGTGGTCAAGGCCTGTGGCTGCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256019
Insert Size	1239 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256019.1, NP_001242948.1
RefSeq Size	2405 bp
RefSeq ORF	1239 bp
Locus ID	12163
Gene Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. Mice lacking a functional copy of this gene exhibit degeneration of germ cells and the epididymal epithelium. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). This isoform (1) may undergo proteolytic processing similar to isoform 2.

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