Fut8 (NM_001252615) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227558 representing NM_001252615 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGATCTATACTACCTCAGTCAAACAGATGGAGCAGGGGATTGGCGTGAAAAAGAGGCCAAAGATC TGACAGAGCTGGTCCAGCGGAGAATAACATATCTCCAGAATCCTAAGGACTGCAGCAAAGCCAGGAAGCT GGTGTGTAACATCAATAAAGGCTGTGGCTATGGTTGTCAACTCCATCACGTGGTCTACTGTTTCATGATT GCTTATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGA CTGTGTTTAGACCTGTAAGTGAGACATGTACAGACAGATCTGGCCTCTCCACTGGACACTGGTCAGGTGA AGTAAATGACAAAAACATTCAAGTGGTCGAGCTCCCCATTGTAGACAGCCTCCATCCTCGGCCTCCTTAC TTACCACTGGCTGTTCCAGAAGACCTTGCAGACCGACTCCTAAGAGTCCATGGTGACCCTGCAGTGTGGT GGGTGTCCCAGTTTGTCAAATACTTGATTCGTCCACAACCTTGGCTGGAAAAGGAAATAGAAGAAGCCAC CAAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCATGTCAGACGCACAGACAAAGTGGGAACAGAA GCAGCCTTCCACCCCATCGAGGAGTACATGGTACACGTTGAAGAACATTTTCAGCTTCTCGCACGCAGAA TGCAAGTGGATAAAAAAAGAGTATATCTGGCTACTGATGATCCTACTTTGTTAAAGGAGGCAAAGACAAA GTACTCCAATTATGAATTTATTAGTGATAACTCTATTTCTTGGTCAGCTGGACTACACAATCGGTACACA GAAAATTCACTTCGGGGTGTGATCCTGGATATACACTTTCTCTCACAGGCTGACTTTCTAGTGTGTACTT TTTCATCCCAGGTCTGTCGGGTTGCTTATGAAATCATGCAAACCCTGCATCCTGATGCCTCTGCGAACTT CCATTCTTTGGATGACATCTACTATTTTGGAGGCCAAAATGCCCACAATCAGATTGCTGTTTATCCTCAC AAACCTCGAACTGAAGAGGAAATTCCAATGGAACCTGGAGATATCATTGGTGTGGCTGGAAACCATTGGG ATGGTTATTCTAAAGGTATCAACAGAAAACTTGGAAAAACAGGCTTATATCCCTCCTACAAAGTCCGAGA GAAGATAGAAACAGTCAAGTATCCCACATATCCTGAAGCTGAAAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252615
Insert Size	1239 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252615.1, NP_001239544.1
RefSeq Size	2086 bp
RefSeq ORF	1239 bp
Locus ID	53618
UniProt ID	Q9WTS2
Gene Summary	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1.

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