Zfp322a (NM_001286155) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Znf322; Znf322a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227547 representing NM_001286155 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACCACTTAGGAGAGAACCATAGAAGATGTACACTTCAGAAGAGGAATGTAACCAGAGAACTCA AAAAAGGAAAACATACCATGTATGCCCTCAAAAGGGTGAAAAAGATTTATATTCGTGTACATGAGATTAC TCAAATAGATAATCAAACATATCAGTGCCTTGAACGTGAACAAAACTTTTGTGAAAACTTAGCACGAATG TGTGAGAGAACATATACCGAGGAGAAACCTTATAGATGTGATATGTGTGAGAAAACCTTCATCCAAAGTT CAGATCTTATTTCCCACCAGAGGATCCATAATTACGAGAAACCTTATAAGTGTAGCAAATGTGAGAAGAG CTTTTGGCACCACTTAGCCCTTTCAGGACACCAGAGAACACATGCAGGTAAAAAGTTTTATACCTGTGAC ATCTGTGGCAAGAATTTTGGTCAGAGCTCTGATCTGCTTGTCCACCAGCGAAGCCATACAGGTGAAAAAC CTTATCTGTGTAATGAGTGTGATAAATGCTTCAGTCGAAGTACAAATCTCATAAGGCACCGAAGAACTCA CACAGGTGAGAAACCGTTTAAGTGTCTGGAATGTGAAAAAGCTTTTAGTGGGAAATCAGATCTTATTAGC CACCAAAGGACTCATACCGGTGAAAGGCCCTACAAATGCAATAAATGTGAGAAAAGTTACCGACACCGGT CTGCCTTCATTGTGCATAAAAGAGTTCATACTGGGGAGAAGCCGTACAAGTGTGGTGCCTGTGAGAAGTG CTTTGGCCAGAAATCAGACCTTATTGTACACCAGAGAGTCCATACAGGAGAGAAACCATATAAATGCTTG GAGTGTATGAGAAGTTTTACTCGAAGTGCCAACCTAATTAGGCACCAGGCAACTCACACTCATACTTTCA AATGCCTTGAATATGAGAAAAGTTTCAACTGTAGCTCAGACTTTATTGTACATCAGAGAATTCATATGGA AGAAAAACCACATCAATGGTCTATGTGTGAGAGTGACTTCCTCCTAGGTATGGACTTCGTTGCCCAGCAG AAAATGAGGGCTCAAACAGAGGAGCTCCATTATAAATACAGTGTATGTGATAAAACCTTTCATCATAGCT CAGCCCTTCTTCAACATCAGACAGTGCACATTGATGATGAATACATCTGTAACATGAGTGAAAAAGGGCT TGATCTAAGTTCTCACGCATCGGAAACCTCACGTGTGTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286155
Insert Size	1233 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286155.1, NP_001273084.1
RefSeq Size	5197 bp
RefSeq ORF	1233 bp
Locus ID	218100
UniProt ID	Q8BZ89
Gene Summary	Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) represents the longest transcript. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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