Casp9 (NM_001277932) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI115399; APAF-3; AW493809; Casp; CASP-9; Caspase-9; ICE-; ICE-LAP6; Mch6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227532 representing NM_001277932 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGCGGACCGGCAGCTCCTGCGGCGATGCAGGGTGCGCCTAGTGAGCGAGCTGCAAGTCGCGG AGCTCTGGGACGCTCTGCTGAGTCGAGAGCTCTTCACGCGCGACATGATCGAGGATATTCAGCAGGCAGG ATCTGGGTCTCGGCGGGATCAGGCCAGGCAGCTGGTCACAGACCTTGAGACCCGAGGGAGGCAGGCCCTT CCTCTCTTCATCTCCTGCTTAGAGGACACAGGCCAAGGCACCCTGGCTTCACTCTTGCAAAGCGGTCGGC AAGCAGCCAAGCAGGATCCAGAGGCTGTTAAACCCCTAGACCACCTGGTGCCTGTGGTCCTGGGACCAAT GGGACTCACAGCAAAGGAGCAGAGAGTAGTGAAGCTGGACCCGTCACAGCCTGCCGTGGGAAACCTCACC CCAGTGGTGCTGGGGCCAGAAGAGCTCTGGCCTGCTCGGCTCAAGCCAGAGGTTCTCAGACCAGAAACAC CCAGGCCCGTGGACATTGGTTCTGGCGGAGCTCATGATGTCTGTGTTCCAGGGAAGATCAGGGGACATGC AGATATGGCATACACCCTGGATTCGGATCCCTGTGGCCACTGCCTCATCATCAACAATGTGAACTTCTGC CCTTCCTCGGGGCTCGGCACACGCACGGGCTCCAACTTGGACCGTGACAAACTTGAGCACCGATTCCGCT GGCTGCGCTTCATGGTGGAGGTGAAGAACGACCTGACTGCCAAGAAAATGGTCACGGCTTTGATGGAGAT GGCACACCGGAACCACCGTGCCCTGGACTGCTTTGTGGTGGTCATCCTCTCTCATGGCTGCCAGGCCAGC CACCTCCAGTTCCCGGGTGCTGTCTATGGGACAGATGGATGCTCCGTGTCCATTGAGAAAATTGTGAATA TCTTCAACGGGAGCGGCTGCCCCAGCCTGGGAGGGAAGCCCAAGCTCTTCTTCATCCAGGCCTGCGGTGG TGAGCAGAAAGACCATGGCTTTGAGGTGGCCTGCACTTCCTCTCAAGGCAGGACCTTGGACAGTGACTCT GAGCCAGATGCTGTCCCCTATCAGGAAGGCCCAAGGCCCTTGGACCAGCTGGATGCTGTGTCAAGTTTGC CTACCCCCAGTGACATCCTTGTGTCCTACTCCACCTTCCCAGGTTGCCAATGCTGTTTCTGCGAAAGGGA CTTACAAGCAGATTCCTGGCTGTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277932
Insert Size	1218 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277932.1, NP_001264861.1
RefSeq Size	3789 bp
RefSeq ORF	1218 bp
Locus ID	12371
Gene Summary	This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (2) lacks an alternate exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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