Vasp (NM_001282021) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227352 representing NM_001282021 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGACGGTCATCTGTTCCAGCCGGGCTACTGTGATGCTTTATGATGACAGCAACAAGCGATGGC TCCCTGCTGGCACTGGTCCCCAGGCCTTCAGCCGCGTCCAGATCTACCACAACCCCACTGCTAACTCCTT CCGAGTTGTTGGCCGCAAGATGCAGCCGGATCAGCAGGTGGTTATCAACTGTGCCATCATTCGGGGTGTC AAGTACAATCAGGCCACTCCCATCTTCCATCAGTGGCGAGATGCCCGCCAGGTCTGGGGCCTCAACTTCG GCAGCAAGGAGGACGCCATACAGTTTGCAACAGGCATGGCCAACGCCCTAGAGGCCTTGGAAGGAGGTGG GCCTCCCCCAGCCCCAGCACCCCCTGCCTGGTCTGCCCAGAATGGTCCCTCCCCAGAGGAGCTGGAACAA CAGAAAAGGCAGCCGGAGCATATGGAGCGCCGGGTCTCCAATGCAGGAGGCCCACCTGCTCCCCCAGCTG GGGGCCCTCCTCCACCTCCAGGACCTCCCCCTCCTCCAGGTCCCCCCCCACCCCCAGGTCTGCCCTCCTC AGGGGTATCTGGGGCAGGTCATGGAGCAGGGGCAGCCCCACCCCCTGCACCCCCACTCCCTACAGCACAG GGCCCCAATAGTGGGGGTTCCGGGGCCCCAGGCCTGGCTGCTGCCATTGCTGGAGCCAAACTCAGGAAAG TGAGCAAGGAGGAGGCCTCTGGGGGGCCCCTGGCCCCCAAAGCTGAGAACAGTCGAAGCACTGGTGGGGG GCTTATGGAAGAGATGAACGCCATGCTGGCCCGGAGAAGAAAAGCCACACAGGTTGGGGAGAAGCCCCCC AAAGACGAGTCAGCCAGTGAGGAGTCAGAGGCCCGACTCCCTGCCCAGAGTGAACCTGTGAGAAGACCCT GGGAGAAGAACAGCACAACCTTGCCAAGGATGAAGTCGTCCTCTTCTGTGACTACCTCCGAGGCCCACCC CTCGACGCCCTGCTCCAGTGATGACTCCGACTTGGAGAGGGTGAAGCAGGAGCTTCTGGAAGAGGTGCGG AAGGAGCTACAGAAAATGAAAGAGGAAATCATCGAAGTCTTTGTCCAGGAGCTGAGGAAGCGGGGTTCTC CTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282021
Insert Size	1125 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282021.1, NP_001268950.1
RefSeq Size	2264 bp
RefSeq ORF	1125 bp
Locus ID	22323
UniProt ID	P70460
Gene Summary	Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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