Nfe2 (NM_001302338) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	NF-E2; NF-E2/P45; p45; p45nf-e2; p45NFE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227340 representing NM_001302338 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCGTGTCCTCCTCAGCAGAACAGGAACAGGTTATCACAGCTGCCTGTTGGGGAGCTTGGAGAGA TGGAACTGACTTGGCAAGAGATCATGTCCATTACTGAGCTGCAGGGTCTAAATGTTCCAAGTGAGACATC TTTTGAGCCTCAAGCACCCACCCCATACCCTGGGCCACTGCCACCTCCAACATATTGCCCCTGTTCAATT CATCCAGATGCAGGCTTCTCCCTTCCCCCACCATCTTATGAGCTCCCAGCATCTACTCCCCATGTCCCAG AACTACCATACTCCTATGGTAATGTAGCCATACCAGTGTCAAAGCCACTTACCCTTTCAGGCCTGCTCAA TGAGCCCCTCCCAGACCACTTAGCTCTCCTGGACATTGGGCTGCCAGTGGGGCAACCCAAGCCCCAAGAA GACCCAGAATCTGACTCAGGATTATCCCTCAACTACAGTGATGCAGAATCTCTTGAGCTAGAGGGTATGG AGGCTGGCAGGCGGAGGAGCGAGTACGCGGACATGTACCCAGTGGAGTATCCTTACTCACTTATGCCCAA TTCTTTGGCCCATCCCAACTATACTCTTCCACCCACTGAGACACCCTTGGCCTTAGAGTCATCCTCCGGT CCAGTTCGGGCTAAGCCTGCTGTCCGTGGGGAGGCAGGGAGTCGGGACGAGCGGCGAGCCCTGGCCATGA AGATTCCTTTCCCTACGGACAAGATAGTTAACTTGCCGGTAGATGACTTTAATGAGTTGTTGGCACAGTA TCCGCTAACGGAGAGCCAGCTGGCTCTAGTTCGGGACATCCGTCGACGGGGCAAGAACAAGGTGGCAGCC CAAAACTGTCGCAAGAGAAAACTGGAAACCATTGTGCAGCTGGAGCGAGAGCTGGAGCGGCTGAGCAGTG AAAGGGAGCGGCTTCTCAGAGCCCGAGGGGAGGCTGACCGCACTCTGGAGGTCATGCGCCAACAGCTGGC AGAGCTGTACCATGATATTTTCCAGCATCTTCGGGATGAATCTGGCAACAGTTACTCACCAGAGGAATAT GTACTGCAACAGGCTGCTGATGGTGCCATCTTTCTGGTACCCCGTGGAACCAAAATGGAGGCTACAGATT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302338
Insert Size	1122 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302338.1, NP_001289267.1
RefSeq Size	1896 bp
RefSeq ORF	1122 bp
Locus ID	18022
UniProt ID	Q07279
Gene Summary	Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3, 4, 5 and 6 encode the same protein.

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