Mdm4 (NM_001302804) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227284 representing NM_001302804 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGTGCAACAGAATACTCCAATCCCAGAAAAAGAACTG AAGAAGAGGATACTCACACACTGCCTACCTCACGACATAAATGCAGAGACTCCAGAGCAGATGAAGACTT GATAGAACATTTATCTCAAGATGAGACATCTAGGCTTGACCTTGATTTTGAGGAGTGGGACGTTGCTGGC CTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAACTGTATTCCTAAAAGTAATGGCTCAACTGATTTAC AGACAAATCAGGATATAGGTACTGCCATTGTTTCAGACACTACGGATGATTTGTGGTTTTTAAATGAGAC CGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGAAGCTGCTAATTCTGAGCAAACAAGTGAAGTAGGG AAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAGGATGATGATCTTGAGGACTCCAGGTCCTTGAGCG ATGATACTGACGTGGAACTTACCTCTGAGGATGAGTGGCAGTGTACGGAATGCAAGAAGTTTAATTCTCC AAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAGAAAGGATTGGTATTCGGATTGTTCTAAATTAACT CATTCCCTATCTACATCTAATATTACTGCCATACCTGAAAAGAAGGACAATGAAGGAATTGATGTTCCCG ATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGCCTAAAGATGGATATTTAAAGGAGGAAAAGCCCAG GTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTTGGCTCATAGTTCTGAAAGCCAGGAGATCATCTCA AGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAGAAAGCTGAAACAGAAAGTATGGAAGATTTCCAGA ATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGCCTCGGGATGGGAACATTATTCATGGGAAGACGAG CCATCTGACGACATGTTTCCACTGTGCCAGGAGACTGAAGAAGTCTGGGGCTTCGTGTCCTGCTTGTAAG AAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302804
Insert Size	1089 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302804.1, NP_001289733.1
RefSeq Size	7194 bp
RefSeq ORF	1089 bp
Locus ID	17248
UniProt ID	O35618
Gene Summary	This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (5) uses an alternate splice site in an internal exon, compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, compared to isoform 1.

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