Wnt5b (NM_001271758) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW545702; Wnt-5b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227267 representing NM_001271758 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGCCTGCTGCTGGTGGTCGTGGCAGCTCTGCTCTCCAGCTGGGCACAGCTGCTGACTGACGCCA ACTCCTGGTGGTCACTAGCTCTGAACCCAGTGCAGAGACCGGAGATGTTCATCATTGGCGCTCAGCCCGT GTGCAGCCAACTTCCTGGGCTTTCCCCAGGCCAGAGAAAGCTGTGTCAGTTGTATCAGGAGCACATGTCC TACATCGGGGAGGGAGCCAAGACGGGCATCAGAGAGTGCCAACACCAGTTTCGACAGAGGCGCTGGAACT GCAGCACCGTGGACAACACATCTGTCTTTGGCAGAGTTATGCAGATAGGTAGCCGAGAGACTGCCTTCAC GTATGCAGTGAGCGCCGCTGGCGTGGTGAATGCCATCAGCCGAGCCTGCAGAGAGGGTGAGCTGTCCACC TGTGGCTGCAGCCGTGCTGCGAGGCCCAAGGACCTGCCTCGGGACTGGCTGTGGGGTGGCTGTGGAGACA ACGTGGAGTACGGCTACCGCTTTGCCAAGGAGTTTGTGGATGCCCGAGAGCGTGAGAAGAACTTTGCCAA GGGATCGGAGGAGCAGGGCCGAGCTCTCATGAACCTACAGAACAACGAGGCTGGCCGCCGGGCCGTGTAT AAGATGGCTGATGTCGCCTGCAAATGTCACGGAGTCTCCGGGTCCTGCAGCCTCAAGACCTGCTGGCTCC AGCTGGCCGAGTTCCGCAAGGTGGGGGACCGTTTGAAGGAGAAGTACGACAGCGCCGCGGCCATGCGCAT CACCCGCCAGGGCAAGCTGGAGCTGGCCAACAGCCGCTTCAACCAGCCCACCCCAGAGGACCTGGTCTAC GTGGACCCCAGTCCTGACTACTGCCTGCGTAATGAGACCACAGGCTCCCTGGGCACCCAGGGTCGCCTCT GCAACAAGACCTCAGAGGGCATGGACGGCTGCGAGCTCATGTGCTGTGGCCGCGGCTATGACCGCTTCAA GAGCGTTCAGGTGGAACGCTGCCACTGCAGGTTCCACTGGTGTTGCTTTGTCAGATGCAAAAAATGCACC GAGGTTGTGGACCAGTATGTCTGTAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271758
Insert Size	1080 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271758.1, NP_001258687.1
RefSeq Size	2117 bp
RefSeq ORF	1080 bp
Locus ID	22419
UniProt ID	P22726
Gene Summary	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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