St3gal3 (NM_001285521) Mouse Untagged Clone

CAT#: MC227256

St3gal3 (untagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 3 (St3gal3), transcript variant 4



  "NM_001285521" in other vectors (1)

CNY 3,420.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Siat3; Siat6; ST3GalIII; ST3N
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227256 representing NM_001285521
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTCCTGGTCCTGGGATTTTTGT
ATTATTCTGCCTGGAAGCTACACTTACTCCAATGGGAAGACTCCAAGTATGATAGGCTGGGCTTCCTCCT
GAAGCTGGACTCTAAACTGCCTGCCGAGCTAGCCACCAAGTACGCAAACTTTTCCGAGGGAGCTTGCAAA
CCCGGCTACGCTTCAGCCATGATGACTGCCATCTTCCCCAGGTTCTCCAAGCCAGCACCCATGTTCCTGG
ATGACTCCTTTCGCAAGTGGGCTAGGATCCGGGAGTTTGTGCCACCTTTTGGGATCAAAGGTCAAGACAA
TCTGATCAAAGCCATCTTGTCAGTCACCAAAGAATACCGCCTGACCCCTGCCTTGGACAGCCTCCACTGC
CGCCGCTGCATCATTGTAGGCAATGGAGGGGTCCTCGCCAACAAGTCTCTGGGGTCACGGATTGACGACT
ATGACATTGTGATCAGACTGAACTCAGCACCTGTGAAGGGCTTTGAGAGGGACGTGGGCAGCAAGACCAC
CCTACGCATCACCTACCCTGAGGGCGCCATGCAGCGACCTGAGCAATATGAACGAGACTCTCTCTTTGTC
CTCGCTGGCTTCAAGTGGCAGGACTTCAAGTGGCTGAAGTACATCGTCTACAAGGAGAGAGTGAGTGCAT
CTGATGGCTTCTGGAAGTCCGTGGCCACCCGAGTGCCCAAGGAGCCCCCTGAGATCCGCATCCTCAACCC
ATACTTCATCCAGGAGGCTGCCTTCACTCTCATTGGACTGCCCTTCAACAACGGCCTCATGGGCAGAGGG
AACATCCCAACCCTTGGCAGTGTGGCAGTGACCATGGCACTACACGGCTGTGATGAAGTGGCAGTCGCGG
GCTTTGGCTATGACATGAACACACCCAATGCACCCCTGCACTACTATGAAACTGTGCGCATGGCAGCCAT
CAAAGAGTCCTGGACACACAACATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTAGTGAAGGCACGTGTC
ATCACTGACTTAAGCAGCGGTATCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001285521
Insert Size 1077 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001285521.1, NP_001272450.1
RefSeq Size 2239 bp
RefSeq ORF 1077 bp
Locus ID 20441
Gene Summary Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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