Bcat2 (NM_001243053) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Bcat-2; Eca40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227230 representing NM_001243053 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAAGAACCACAGAAGAAGCCAGCCCCTAGCCAGGCACTGCTGTTTGGGAAGACCTTCACAGACC ACATGCTGATGGTGGAGTGGAATAACAAGGCTGGATGGGGCCCACCAAGGATCCAGCCCTTCCAGAACCT CACGCTACACCCAGCCTGCTCTGGTCTGCACTACTCTCTGCAGCTCTTTGAGGGCTTGAAAGCATACAAA GGTGGAGACCAGCAGGTACGCCTCTTCCGGCCCTGGCTCAACATGGACAGGATGCTACGTTCTGCTAGGC GCCTCTGCCTGCCAGACTTTGACAAGCAGGAGCTGTTGGAGTGTATCCGCCAGCTCATTGAAGTAGACAA AGACTGGGTTCCCGATGGCAACGGAACGAGCCTCTACGTGCGGCCCGTGCTGATCGGGAATGAGCCCTCG CTGGGTGTTGGTATGGTCACACAAGCCCTCCTGTACGTCATTCTGTGCCCTGTGGGCTCCTACTTCCCTG GAGACTCCATGACACCTGTCTCCCTCCTGGCGGACCCTTCATTCGTCAGAGCCTGGATAGGCGGAGTCGG TGACTGCAAGTTGGGGGGGAACTATGGACCCACTGTGGCTGTGCAGCGAGAAGCCCAGAAGAGAGGCTGC GAGCAGGTCCTCTGGCTGTACGGGCCAGACCACCAGCTCACTGAGGTGGGCACCATGAACATCTTTGTCT ACTGGACCCATGAAGACGGAGTACTGGAGCTGGTAACCCCCCCACTGAATGGCGTCATCTTGCCTGGAGT AGTTCGACAAAGTCTGCTGGATCTGGCCAGGACTTGGGGTGAGTTCCGGGTGGCAGAACGCAAGGTCACT ATGAAGGAATTGAAGCGGGCGCTGGAGGAAGGCCGAGTGCGAGAAGTCTTCGGCTCAGGCACTGCTTGTC AGGTCTGCCCGGTGCACCAAATCCTGTATGAAGGCAAGCAACTCCACATACCTACCATGGAGAACGGCCC AGAGCTCATCCTGCGCTTCCAGAAGGAACTGAAGGCTATTCAGTACGGAGCCAGTGCCCACGATTGGATG TTTCGGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243053
Insert Size	1062 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243053.1, NP_001229982.1
RefSeq Size	1692 bp
RefSeq ORF	1062 bp
Locus ID	12036
Gene Summary	Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.[UniProtKB/Swiss-Prot Function]

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