Mef2a (NM_001291196) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430079H05Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227222 representing NM_001291196 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTCACAGTCCCTGTGACCAGCCCTAATGCTTTGTCGTACACTAACCCAGGGAGTTCACTCGTGT CACCGTCTTTGGCAGCCAGCTCAACATTAGCAGATTCGAGCATGCTGTCTCCACCCCCAGCCACGCTACA TAGAAATGTGTCCCCAGGAGCTCCGCAGAGACCACCAAGTACCGGCAGTGCAAGTGGGATGTTGAGCACT ACAGACCTCACGGTGCCAAATGGAGCTGGAAATAGTCCTGTGGGGAATGGATTTGTAAACTCAAGGGCCT CTCCAAATTTGATTGGAAATACTGGTGCAAATAGTTTAGGCAAAGTCATGCCTACAAAGTCTCCCCCGCC ACCAGGTGGTGGCAGTCTTGGAATGAACAGTCGGAAACCAGATCTACGAGTTGTCATTCCCCCATCAAGC AAGGGCATGATGCCCCCACTTAATGCCCAAAGGATAAGCAGTTCTCAAGCCACTCAACCTCTTGCCACGC CTGTAGTGTCTGTGACAACTCCGAGCTTGCCTCCACAGGGACTAGTGTACTCAGCAATGCCGACAGCCTA CAACACTGATTACTCACTGACCAGCGCTGACCTGTCTGCCCTGCAAGGCTTTACTTCCCCTGGAATGCTG TCTCTGGGGCAGGCTTCAGCCTGGCAGCAGCACCATCTAGGACAAGCAGCCCTCAGCTCTCTGGTGGCTG GAGGGCAGTTATCTCAGGGTTCAAATTTGTCCATTAACACCAACCAGAACATCAACATTAAGTCAGAGCC AATTTCACCTCCCCGTGATAGAATGACCCCATCAGGCTTCCAACAGCAGCAGCAGCAGCCACAACAGCAG CCACCGCCACAACCTCCACAGCCCCAACCCCGGCAGGAAATGGGGCGCTCCCCTGTGGACAGTCTGAGCA GCTCCAGCAGCTCCTATGATGGCAGTGACCGGGAGGATCCACGGGGTGACTTCCATTCTCCAATTGTGCT TGGCCGACCTCCAAATACTGAGGACAGAGAAAGCCCTTCTGTAAAACGCATGAGGATGGACACATGGGTG ACCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291196
Insert Size	1056 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291196.1, NP_001278125.1
RefSeq Size	5675 bp
RefSeq ORF	1056 bp
Locus ID	17258
UniProt ID	Q60929
Gene Summary	Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) contains an alternate exon, lacks an alternate in-frame exon, and initiates translation at an alternate start codon compared to variant 1. The resulting isoform (d) is shorter at the N-terminus and lacks an internal segment compared to variant 1.

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