Sae1 (NM_001285891) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2400010M20Rik; 2610044L12Rik; AL033372; AOS1; AW743391; D7Ertd177e; HSPC140; Sua1; Uble1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227211 representing NM_001285891 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGAGAAGGAGGAGGCTGGCGGCGGCGGCGGCGGTGGCATCAGCGAGGAGGAGGCAGCGCAGTATG ACCGACAGATCCGCCTGTGGGGACTGGAGGCCCAGAAGCGGCTTCGAGCTTCCCGGGTGCTGATTGTTGG CATGAAAGGACTTGGAGCTGAAATTGCCAAGAATCTTATCCTGGCAGGAGTCAAAGGGCTCACCATGCTG GACCACGAACAGGTATCTCCAGAAGACCCTGGAGCTCAGTTCTTGATTCAGACTGGGTCTGTGGGCCGCA ATAGGGCCGAGGCCTCTTTGGAACGAGCCCAGAATCTCAATCCCATGGTGGATGTAAAGGTGGACACTGA GGATGTAGAGAAGAAGCCAGAGTCCTTCTTCACGAAGTTTGATGCGGTGTGCCTGACTTGCTGCTCCAGA GATGTCATCATTAAAGTCGACCAGATCTGTCACAGAAACAGCATTAAGTTCTTCACAGGAGATGTCTTTG GGTACCATGGGTACACCTTTGCGAATCTTGGAGAGCATGAATTTGTAGAAGAGAAAACCAAAGTTGCCAA AGTAAGCCAAGGAGTGGAAGATGGACCTGAGGCCAAGAGAGCAAAGCTTGATTCATCAGAGACAACCATG GTCAAAAAGAAAGTGCTTTTTTGCCCTGTAAAAGAGGCGCTAGAAGTGGACTGGAGTGGAGAGAAAGCCA AGGCTGCCCTGAAGCGTACTGCCCCCGACTACTTTCTGCTGCAAGTGCTGCTGAAATTCCGCACAGATAA AGGGAGAGATCCGACTTCTGAGAGCTACAAGGAGGATGCGGAGCTGCTGCTGCAGATACGGAATGACGTG TTTGACTCCCTGGGCATCAGTCCTGACCTGCTTCCCGATGACTTTGTCAGGTACTGCTTCTCTGAGATGG CCCCAGTATGTGCTGTGGTTGGAGGGATCTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAAAGGGACCC TCCTCACAACAACTTCTTCTTCTTCGACGGCATGAAGGGGAGTGGAATTGTGGAGTGCCTTGGTCCCCAG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285891
Insert Size	1053 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285891.1, NP_001272820.1
RefSeq Size	1850 bp
RefSeq ORF	1053 bp
Locus ID	56459
UniProt ID	Q9R1T2
Gene Summary	The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).

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