Fbxl12 (NM_001286529) Mouse Untagged Clone

CAT#: MC227201

Fbxl12 (untagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 3



  "NM_001286529" in other vectors (1)

CNY 3,420.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3110048D16Rik; Fbl12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227201 representing NM_001286529
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGGGACTTGCTAAGAATGGATTTACAGCGGACGTTGGAACTGGTGTTGGAGATTCCTTCATCAT
GGAAGAAACCTAAAGGATCTCAGCGGCGGGGTGCTCGACTACTCCCAAGCCAGACAATCCTCCTACCGCG
GAATCCCGGCTGCAGGGTCTGTCACCGCTGGAAGAGGCTAGTGGATGACCGGTGGCTATGGCGACACGTC
GACCTGACGCTCTACACGATGCGGCCGAAAGTCATGTGGCACCTCCTGCGCCGGTACATGGCGTCCCGGC
TCTACTCGTTGCGCATGGGCGGCTACCTGTTTTCTGGCTCTCAGGCCCCACAGCTGTCCCCCGCCTTGAT
GAGGGCCCTGGGTCAGAAATGCCCCAATCTGAAGCGCCTGTGCCTGCACGTGGCTGACCTGAGCATGGTG
CCTATCACCAGCCTTCCTAGCACACTGAGGACCCTGGAGCTGCACAGCTGCGAAATCTCCATGATCTGGT
TGCAGAAAGAGCAGGACCCCACGGTGCTGCCTCTGCTGGAATGCATCGTGCTGGACCGAGTGCCGGCCTT
TCGCGATGAGCATCTGCAGGGCCTTACCCGATTCCGAGCCCTGCGCTCGCTGGTGCTGGGCGGCACCTAC
CGGGTCACTGAGACCGGGCTAGATGCCAGCCTGCAGGAGCTCAGCTACCTGCAAAGGCTTGAGGTGCTGG
GCTGCACCCTGTCAGCTGACAGCACGCTGCTGGCCATCAGCCGCCACCTTCGAGATGTGCGCAAGATTCG
GCTGACCGTTGGGGGCCTCTCAGCCCAGGGCCTGGTCTTCCTGGAGGGAATGCCTGTCCTGGAGAGTTTG
TGCTTCCAGGGTCCCCTTATCACCCCAGACATGCCCACACCCACTCAGATTGTGTCCTCCTGCCTCACCA
TGCCGAAGCTCAGAGTGCTTGAGGTGCAAGGGCTGGGCTGGGAGGGTCAGGAAGCAGAGAAAATCCTGTG
CAAGGGCCTGCCCCACTGCATAGTTATTGTTAGGGCTTGTCCCAAAGAATCCATGGATTGGTGGATGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001286529
Insert Size 1050 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286529.1, NP_001273458.1
RefSeq Size 1718 bp
RefSeq ORF 1050 bp
Locus ID 30843
UniProt ID Q9EPX5
Gene Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses an alternate 5' structure and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform c, which has a longer and distinct N-terminus, compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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