Ctss (NM_001267695) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cats
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227140 representing NM_001267695 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCTCCTGGCCACGCTGCCATCAGAAGATGGCTGTTTTGGATGCCCCTGGTGTGCTCTGTGGCAA TGGAGCAACTGCAGAGAGACCCTACCCTGGACTACCATTGGGATCTCTGGAAGAAAACCCATGAGAAAGA ATACAAGGATAAGAATGAAGAAGAAGTACGGCGTCTCATCTGGGAAAAGAATCTGAAGTTTATAATGATT CACAATCTCGAATATTCTATGGGAATGCATACCTACCAAGTGGGCATGAACGATATGGGAGACATGACCA ATGAAGAAATCTTGTGTCGGATGGGTGCTCTGAGAATTCCCCGACAGTCTCCGAAGACTGTCACTTTCAG GTCATACTCTAATCGGACATTGCCTGACACTGTGGACTGGAGAGAGAAGGGCTGCGTCACTGAGGTGAAA TACCAGGGTTCTTGTGGTGCCTGCTGGGCTTTCAGTGCTGTGGGGGCCCTTGAAGGGCAGCTGAAGCTGA AAACGGGGAAGCTGATATCCCTCAGTGCTCAGAACCTGGTGGACTGCTCAAATGAAGAAAAGTATGGGAA TAAAGGCTGTGGAGGCGGCTACATGACCGAAGCTTTCCAGTACATCATTGATAATGGGGGCATAGAGGCA GACGCTTCCTATCCCTACAAAGCCACGGATGAAAAGTGTCACTATAACTCAAAAAATCGAGCTGCCACGT GTTCAAGGTACATTCAGCTCCCGTTTGGTGACGAGGATGCCCTGAAAGAAGCAGTGGCCACTAAAGGGCC TGTCTCTGTGGGCATCGACGCCAGCCATTCCTCCTTCTTCTTCTACAAAAGCGGTGTCTATGACGACCCC TCCTGTACGGGCAATGTGAATCATGGTGTTCTTGTGGTTGGCTATGGGACTCTTGATGGCAAAGATTACT GGCTTGTGAAAAACAGTTGGGGCCTTAACTTTGGTGATCAAGGATACATTCGGATGGCAAGAAATAATAA AAATCACTGCGGAATTGCTAGTTATTGCTCTTACCCAGAAATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001267695
Insert Size	1026 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267695.2, NP_001254624.2
RefSeq Size	1360 bp
RefSeq ORF	1026 bp
Locus ID	13040
Gene Summary	This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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