Mbnl1 (NM_001253708) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Mbnl; mKIAA0428
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227137 representing NM_001253708 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTTAGTGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTTCAAA GGGGGACTTGCTCACGACCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGTTGAAAA TGGACGAGTAATCGCCTGCTTTGATTCACTGAAAGGTCGTTGCTCCAGAGAGAACTGCAAATATCTTCAT CCACCCCCACACTTAAAAACACAGTTAGAGATAAATGGGCGGAATAACTTGATTCAGCAGAAGAACATGG CCATGCTGGCCCAGCAAATGCAGTTAGCCAATGCCATGATGCCCGGTGCCCCGTTGCAGCCCGTGCCAAT GTTTTCAGTTGCACCAAGCTTAGCCACCAGTGCATCAGCAGCCTTTAACCCTTACCTGGGGCCTGTTTCC CCAAGCCTGGTTCCAGCAGAGATCTTGCCGACTGCACCAATGTTGGTCACGGGGAATCCTGGAGTTCCAG TGCCAGCAGCTGCCGCAGCTGCTGCACAGAAGTTAATGCGGACAGACAGACTGGAGGTGTGTCGAGAGTA CCAGCGTGGCAATTGCAACAGAGGAGAAAATGACTGTCGGTTTGCTCATCCTGCTGACAGCACAATGATT GATACCAATGACAACACAGTCACTGTCTGCATGGATTACATCAAGGGGAGATGCTCTCGGGAAAAGTGCA AATACTTCCATCCTCCCGCACACCTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGC AGCAGCACAGGCTGCAGCTACTGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTGCCAAAG AGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGG CTCTAGCCAACATGCAGTTACAGCAGCATACAGCATTTCTCCCACCAGGCTCAATATTGTGCATGACACC CGCTACAAGTGTTGATACCCATAATATCTGCCGAACATCTGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253708
Insert Size	1026 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253708.2, NP_001240637.1
RefSeq Size	5578 bp
RefSeq ORF	1026 bp
Locus ID	56758
UniProt ID	Q9JKP5
Gene Summary	Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon near the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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