Nfyc (NM_008692) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227100 representing NM_008692 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACAGAAGGAGGGTTTGGCGGTACCAGCAGCAGTGATGCCCAGCAAAGCCTCCAGTCCTTCTGGC CCAGAGTCATGGAAGAAATCCGAAACTTAACAGTGAAAGATTTCCGAGTACAGGAACTCCCACTGGCTCG TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCCCCTGTGCTGTTTGCT AAGGCAGCCCAGATTTTTATCACTGAGCTGACTCTTCGAGCCTGGATCCACACAGAGGATAACAAGCGTC GTACTCTTCAGAGGAATGATATTGCCATGGCAATTACAAAATTTGATCAGTTCGACTTTCTCATCGATAT TGTTCCAAGAGATGAACTGAAACCTCCAAAGCGCCAGGAGGAGGTACGCCAGTCTGTGACTCCTGCGGAG CCTGTCCAGTACTACTTCACGCTGGCTCAGCAGCCCACTGCTGTCCAGGTCCAGGGACAGCAGCAAGGCC AGCAGACCACCAGTTCTACGACCACCATCCAGCCTGGACAGATCATCATTGCACAGCCTCAGCAGGGTCA GACCACACCCGTGACCATGCAGGTTGGAGAAGGTCAGCAGGTGCAGATTGTACAGGCCCAACCTCAGGGT CAGGCCCAGCAGACCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATTACCAACACAG GAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCTGGCCAGCTGCAATATATCCGATTAGCCCAGCCTGT ATCAGGCACCCAAGTTGTCCAAGGACAGATCCAGACACTTGCTACCAATGCCCAACAGATCACACAGACA GAGGTCCAGCAAGGACAGCAGCAGTTCAGCCAGTTCACAGATGGACAGCAGCTGTACCAGATCCAGCAAG TCACCATGCCTGCAGGCCAAGACCTCGCCCAGCCCATGTTTATTCAGTCAGCCAATCAGCCCTCTGATGG GCAGACCCCCCAGGTGACTGGAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_008692
Insert Size	1008 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008692.4, NP_032718.2
RefSeq Size	1990 bp
RefSeq ORF	1008 bp
Locus ID	18046
UniProt ID	P70353
Gene Summary	Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the llonger isoform (1). Variants 1, 2, 4, 5 and 6 encode the same isoform (1).

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