Gfra2 (NM_001302095) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227079 representing NM_001302095 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTTGGCAAACGCCTTCTGCCTCTTCTTCTTTTTAGGGACAGGGGCAGACCCGGTGGTCAGTGCCA AGAGCAACCACTGCCTGGATGCCGCCAAGGCCTGCAACCTGAACGACAACTGCAAGAAGCTCCGCTCCTC CTACATCTCCATCTGCAACCGCGAGATCTCTCCCACCGAACGCTGCAACCGCCGCAAGTGCCACAAGGCC CTGCGCCAGTTCTTCGACCGTGTGCCCAGCGAGTATACCTACCGCATGCTCTTCTGCTCCTGTCAGGACC AGGCATGCGCCGAGCGTCGCCGGCAAACCATCCTGCCCAGCTGTTCCTATGAGGACAAAGAGAAGCCCAA TTGCTTGGACCTGCGCAGCCTGTGTCGTACAGACCACTTGTGCCGGTCCCGCCTGGCAGACTTCCACGCC AACTGTCGAGCCTCCTACCGGACAATCACCAGCTGCCCTGCGGACAACTACCAGGCATGTCTGGGCTCCT ATGCTGGCATGATTGGGTTTGATATGACACCGAACTATGTGGACTCCAACCCCACGGGCATCGTGGTGTC TCCCTGGTGCAATTGTCGTGGCAGTGGGAACATGGAAGAAGAGTGTGAGAAGTTCCTCAAGGACTTCACA GAAAACCCATGCCTCCGGAATGCCATTCAAGCCTTTGGCAATGGCACAGATGTGAACATGTCTCCCAAAG GCCCCACATTTTCAGCTACCCAGGCCCCTCGGGTAGAGAAAACTCCTTCACTGCCAGATGACCTCAGTGA TAGCACCAGCCTGGGGACCAGTGTCATCACCACCTGCACATCTATCCAGGAGCAAGGGCTGAAGGCCAAC AACTCCAAAGAGTTAAGCATGTGTTTCACAGAGCTCACGACAAATATCAGCCCAGGGAGTAAAAAGGTGA TCAAACTTTACTCAGGCTCCTGCAGAGCCAGACTGTCGACTGCCTTGACTGCCCTCCCACTCCTGATGGT GACCTTGGCCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302095
Insert Size	996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302095.1, NP_001289024.1
RefSeq Size	3088 bp
RefSeq ORF	996 bp
Locus ID	14586
UniProt ID	O08842
Gene Summary	The protein encoded by this gene is part of the receptor complex that transduces glial cell-derived neurotrophic factor and neurturin signals by mediating autophosphorylation and activation of the RET receptor. Mice lacking this protein are viable and fertile but display growth retardation attributed to impaired salivary and pancreatic secretion and innervation deficits in the intestinal tract. In addition, knockout mice display neural defects including a failure to initiate outgrowth of dorsal ganglion root neurons, demonstrating a requirement in neuronal differentiation of these cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) lacks two consecutive in-frame exons in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1.

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