Ewsr1 (NM_001283063) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ews; Ewsh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227041 representing NM_001283063 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCACGGATTACAGTACCTATAGTCAAGCTGCAGCCCAGCAGGGCTACAGTGCTTACACCGCCC AGCCAACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCC TACTGATGTCAGCTATACTCAGGCTCAGACCACTGCCACCTACGGGCAGACTGCATATGCAACTTCTTAC GGACAGCCTCCCACTGGTTATAGCACTCCAACTGCCCCGCAGGCGTACAGCCAGCCTGTGCAGGGATATG GCACTGGGACTTATGACAGCACCACTGCTACAGTCACCACAACGCAGGCCTCTTACGCAGCTCAGACTGC ATATGGCACCCAGCCTGCCTACCCCACCTATGGCCAGCAGCCAACAGCCACCGCACCTACCAGACCACAG GATGGTAACAAGCCTGCTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTATAACCAACCCAGCCTAG GATATGGACAGAGTAACTACAGCTATCCCCAGGTACCTGGGAGCTACCCAATGCAGCCAGTCACCGCACC TCCATCTTATCCTCCTACCAGCTACTCCTCTTCACAGCCGACTAGTTACGATCAGAGCAGTTACTCTCAG CAGAACACCTATGGGCAGCCGAGCAGCTATGGACAACAGAGTAGCTATGGTCAACAAAGCAGCTATGGGC AGCAGCCTCCTACTAGTTACCCCCCTCAGACTGGATCCTACAGCCAGGCTCCAAGTCAATATAGCCAACA GAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAG GAGTCTGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCTTGAGTGGCCCTGATAACCGGGGCAGGGGAA GAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGGAGGAGGACGCGGTGGACTGGGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001283063
Insert Size	978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001283063.1, NP_001269992.1
RefSeq Size	3216 bp
RefSeq ORF	978 bp
Locus ID	14030
Gene Summary	Might function as a transcriptional repressor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (4) is shorter at the C-terminus compared to isoform 1.

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