Whrn (NM_001276371) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110035G07Rik; AW122018; AW742671; C430046P22Rik; Dfnb3; Dfnb31; Ush2; Ush2d; w; wi
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227028 representing NM_001276371 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGCACAGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCGGCGG CGGCGGGCGGCGGCGGGGGCGCGGGGTTGCGGCTGCTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCT CACGGCGCTGCTGAGCGAGCCCGAGCGGGAGCAGTTCACTCACTGCCTCAACGCGTACCACGCGCGCCGC AACGTCTTCGACCTGGTGCGCACCCTGCGCGTCCTGCTGGACAGCCCGGTCAAGCGGCGTCTGCTGCCCA TGCTGCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAGTACACGGCCGAGGGCCTCTACCT GCCAGCCACCACCCCCTACAGGCAGCCCGCCTGGGCCGCTCCCGACGGCGCGGGGCCCGGGGAGGTGCGA CTCGTGAGCCTGCGGCGCGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGCGGGGGCTCGGAACACG GCGTGGGCATCTACGTGTCTCTAGTGGAGCCGGGCTCCCTGGCAGAGAAGGAAGGGTTGCGGGTCGGGGA CCAGATTCTGCGCGTCAACGATAAATCTCTAGCCCGGGTGACCCACGCGGAGGCTGTCAAGGCTCTCAAA GGCTCCAAGAAGCTGGTGCTGTCTGTATACTCAGCTGGGCGTATCCCAGGGGGCTATGTGACCAACCACA TCTACACCTGGGTGGACCCACAGGGTCGAAGCACATCCCCTCCCTCCAGCCTGCCCCAGCCCCATGGCAG CACCCTGAGACAGCGTGAAGATGACCGAAGGAGTACCCTCCACCTCCTGCAGAGTGGAGATGAGAAAAAG GTGAACCTGGTGTTGGGGGACGGCCGGTCCTTGGGCCTCACGATCCGAGGTGGAGCAGAGTACGGCCTTG GCATTTACATCACTGGTGTGGACCCAGGCTCTGAAGCAGAGAGCAGCGGCCTCAAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001276371
Insert Size	969 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276371.1, NP_001263300.1
RefSeq Size	2040 bp
RefSeq ORF	969 bp
Locus ID	73750
UniProt ID	Q80VW5
Gene Summary	This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013] Transcript Variant: This variant (10) uses an alternate 3' terminal exon, compared to variant 2. It encodes isoform 10 which is shorter and has a distinct C-terminus, compared to isoform 2.

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