Lgals8 (NM_001199043) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200015E08Rik; AI326142; D13Ertd524e; Lgals-8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226987 representing NM_001199043 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCCTTAAATAACCTACAAAATATCATCTATAACCCGATAATCCCCTATGTTGGCACCATTACTG AGCAATTGAAGCCTGGCTCTCTGATTGTAATCCGTGGGCATGTCCCTAAAGATTCAGAAAGATTCCAGGT TGACTTTCAGCTGGGCAACAGCCTGAAGCCAAGAGCAGACGTGGCCTTCCACTTTAACCCTCGGTTCAAA AGGTCTAGCTGCATTGTTTGTAACACACTGACACAGGAGAAGTGGGGCTGGGAGGAGATCACCTACGACA TGCCCTTCAGAAAAGAAAAGTCCTTTGAGATCGTGTTCATGGTGCTCAAGAACAAATTCCAGGTGGCTGT GAACGGAAGGCATGTTCTGCTGTACGCCCACAGGATCAGCCCGGAGCAGATCGACACAGTGGGCATCTAC GGCAAAGTGAACATCCACTCCATCGGGTTCAGATTCAGCTCGGATTTACAGAGTATGGAAACATCTGCTC TGGGACTGACACAGATAAACAGAGAGAATATACAAAAGCCAGGCAAGCTCCAGCTGAGCCTGCCATTTGA AGCAAGGTTGAATGCCTCCATGGGTCCTGGACGAACCGTTGTCATTAAAGGGGAAGTGAACACCAATGCC CGAAGCTTTAATGTTGACCTAGTGGCAGGAAAAACAAGGGATATCGCTCTGCACTTGAACCCACGCCTCA ATGTGAAAGCATTTGTAAGAAATTCCTTTCTTCAGGATGCCTGGGGAGAAGAGGAGAGAAATATTACCTG CTTCCCATTTAGTTCTGGGATGTACTTTGAGATGATAATCTACTGTGATGTCCGGGAATTCAAGGTTGCT ATAAATGGTGTGCACAGCCTGGAGTACAAACACAGATTTAAAGACCTAAGCAGTATTGATACACTATCAG TCGATGGTGATATCCGTTTGCTGGATGTAAGGAGCTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199043
Insert Size	951 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199043.1, NP_001185972.1
RefSeq Size	2996 bp
RefSeq ORF	951 bp
Locus ID	56048
UniProt ID	Q9JL15
Gene Summary	Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 3. Variants 1 and 2 encode the same isoform (1), which is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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