Eno2 (NM_001302642) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI837106; D6Ertd375e; Eno-2; NSE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226984 representing NM_001302642 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTATAGAGAAGATTTGGGCCCGAGAGATCTTGGACTCCCGTGGGAATCCCACCGTGGAGGTGGATC TCTATACTGCCAAAGGTCTTTTCCGGGCTGCAGTCCCCAGTGGAGCCTCCACCGGCATCTATGAGGCCCT GGAACTAAGGGATGGGGACAAACAGCGTTACTTAGGCAAAGGTGTCCTGAAGGCAGTGGACCACATCAAC AGCAGGATTGCACCAGCCCTCATCAGCTCAGGTATCTCCGTGGTGGAGCAGGAGAAACTGGACAACCTGA TGTTGGAACTGGATGGGACTGAGAATAAATCCTTGGAGCTGGTGAAGGAAGCCATCGACAAGGCTGGCTA CACGGAAAAGATGGTGATCGGTATGGATGTGGCTGCCTCTGAGTTTTACCGCGATGGCAAATACGACTTG GATTTCAAGTCTCCCGCTGATCCTTCCCGATACATCACTGGGGACCAGCTGGGGGCACTCTACCAGGACT TTGTCCGGAACTATCCTGTGGTCTCCATTGAAGACCCATTTGACCAGGACGACTGGGCAGCTTGGTCCAA GTTCACAGCCAACGTCGGCATCCAGATAGTGGGCGATGACCTGACGGTGACCAACCCCAAGCGCATCGAG CGGGCAGTGGAGGAGAAGGCCTGCAACTGTTTGCTGCTCAAGGTCAATCAGATCGGCTCGGTCACAGAAG CCATCCAAGCGTGCAAGCTGGCCCAGGAGAATGGCTGGGGGGTTATGGTGAGCCATCGCTCTGGAGAAAC GGAGGACACGTTCATTGCTGATCTTGTCGTCGGACTGTGTACAGGCCAGATCAAGACTGGCGCCCCATGC AGATCTGAACGTCTGGCGAAGTACAACCAGCTCATGAGAATTGAGGAAGAGCTGGGGGACGAAGCTCGCT TCGCGGGACATAATTTCCGAAATCCCAGTGTGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302642
Insert Size	948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302642.1, NP_001289571.1
RefSeq Size	2454 bp
RefSeq ORF	948 bp
Locus ID	13807
UniProt ID	P17183
Gene Summary	Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two in-frame exons in the coding region compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.

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