Zap70 (NM_001289612) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mrtle; mur; S; Srk; ZAP-; ZAP-70
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226959 representing NM_001289612 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATGGACACAAGTGTGTACGAGAGTCCCTACAGCGACCCTGAGGAACTCAAAGACAAGAAGCTCT TCCTGAAGCGAGAGAATCTCCTCGTGGCGGACATCGAGCTTGGCTGTGGCAACTTTGGCTCCGTGCGCCA GGGAGTCTATCGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGGCACAGAGAAG GCCGACAAAGATGAGATGATGCGAGAGGCCCAGATCATGCACCAGCTCGACAACCCCTACATCGTGCGGC TCATCGGCGTGTGCCAGGCAGAAGCACTCATGCTGGTCATGGAGATGGCGGGAGGCGGGCCCCTGCACAA GTTCCTGCTGGGAAAGAAGGAGGAGATCCCTGTGAGCAATGTGGCTGAACTGCTGCACCAGGTGGCCATG GGCATGAAGTATTTGGAGGAGAAAAACTTTGTGCACCGCGACCTGGCAGCCCGCAATGTTCTACTGGTCA ATCGGCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAAGCCCTGGGTGCTGACGACAGCTATTACAC AGCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGAAGTTCTCC AGCCGCAGTGACGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAGAAGCCCTACA AGAAAATGAAGGGCCCCGAGGTCCTGGACTTCATCAAGCAGGGTAAGAGGATGGAATGTCCGCCGGAGTG TCCTCCTGAGATGTATGCACTTATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCCCCGACTTCCTG ACTGTGGAACAACGTATGCGGAACTATTACTACAGCCTGGCCAGCCGGGCCGAGGGACCCCCACAGTGTG AACAGGTGGCCGAGGCTGCATGTGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289612
Insert Size	939 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289612.1, NP_001276541.1
RefSeq Size	1343 bp
RefSeq ORF	939 bp
Locus ID	22637
UniProt ID	P43404
Gene Summary	This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) contains a 5' terminal exon that extends past a splice site that is used in variant 1. This variant differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 4. The encoded isoform (TZK, also known as truncated ZAP kinase; PMID 14985102) has a shorter N-terminus compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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