Tomm34 (NM_001291155) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610100K07Rik; TOM34
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226942 representing NM_001291155 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCAAAGTCTCGGATTCCGTGGAACAGCTCCGCGCTGCCGGCAACCAGAACTTCCGCAATGGCC AGTACGGCGAAGCCTCGGCGCTGTACGAGCGCGCACTGCGGCTGCTGCAGGCGCGAGGTTCTGCAGACCC CGAAGAAGAAAGTGTTCTGTACTCCAACCGTGCAGCGTGCTACTTGAAGGATGGGAACTGCACAGATTGC ATCAAAGATTGCACTTCCGCGCTGGCCTTGGTTCCCTTCAGCATCAAGCCCTTGCTGCGCAGAGCATCTG CATATGAAGCCCTGGAGAAGTACGCCCTGGCCTACGTTGACTATAAGACTGTGCTGCAGATCGATAACAG TGTGGCATCCGCCCTGGAAGGCATCAACAGAATAACCAGAGCTCTCATGGACTCCCTGGGACCTGAGTGG CGCCTGAAGCTGCCCCCTATCCCTGTGGTGCCTGTTTCAGCCCAGAAGAGATGGAATTCCTTGCCTTCAG ATAACCACAAAGAGACAGCTAAAACCAAATCCAAAGAAGCCACAGCTACGAAGAGCAGAGTGCCTTCTGC TGGGGATGTGGAGAGAGCCAAAGCTCTGAAGGAAGAAGGCAATGACCTTGTAAAGAAGGGCAACCATAAG AAAGCTATTGAGAAGTACAGTGAGAGCCTCTTGTGTAGTAGCCTGGAGTCTGCCACATACAGCAACAGAG CGCTCTGTCACCTGGTCCTGAAGCAGTACAAGGAGGCAGTAAAGGACTGCACAGAAGCCCTCAAGCTGGA TGGGAAGAATGTAAAGGCGTTTTACAGACGGGCTCAAGCCTACAAGGCACTCAAGGACTATAAGTCAAGC CTTTCGGATATCAGCAGCCTCCTACAAATTGAACCCAGGAATGGCCCTGCACAGAAGTTACGGCAGGAAG TTAACCAGAACATGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291155
Insert Size	930 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291155.1, NP_001278084.1
RefSeq Size	1940 bp
RefSeq ORF	930 bp
Locus ID	67145
UniProt ID	Q9CYG7
Gene Summary	Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (Tom34a) contains an alternate 5' terminal exon and it thus differs in the 5' UTR and 5' coding region, compared to variant Tom34b. The encoded isoform (a, also known as Tomm34a) has a distinct N-terminus and is the same length as isoform b.

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