Crem (NM_001271505) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	IC; ICER; ICERI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226934 representing NM_001271505 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAATGTGGCAGGAAAAAGTATATGAGGACAAATGTAAGGCAAATGACCATGGAAACAGTTGAAT CACAGCAGGATCGAAGTGTAACACGTTCTGTGGCAGAGCATAGCTCTGCTCATATGCAGACTGGTCAAAT TTCTGTTCCTACTCTAGCTCAGGTAGCAACAATTGCAGAGACAGATGATTCTGCAGACTCAGAAGTAATT GATTCGCATAAACGTAGAGAAATTCTTTCACGAAGACCCTCATATAGAAAAATACTGAATGAACTTTCCT CTGATGTGCCTGGTATTCCCAAGATTGAAGAAGAAAAATCAGAGGAAGAAGGGACACCACCTAACATTGC TACCATGGCAGTACCAACTAGCATATATCAGACTAGCACGGGGCAATACATTGCTATAGCTCAAGGTGGA ACAATCCAGATTTCTAACCCAGGATCTGATGGTGTTCAGGGACTCCAGGCATTAACAATGACAAATTCAG GAGCTCCTCCGCCAGGTGCTACAATTGTACAGTATGCAGCACAATCAGCCGATGGTACACAGCAGTTCTT TGTCCCAGGCAGCCAGGTTGTTGTTCAAGATGAGGAGACTGACCTTGCCCCAAGTCACATGGCTGCTGCC ACAGGTGACATGCCAACTTACCAGATCCGAGCTCCTACTACTGCTTTGCCACAAGGTGTGGTGATGGCTG CCTCACCAGGAAGCCTGCACAGTCCCCAGCAACTAGCAGAAGAAGCAACTCGCAAGCGGGAGCTGAGGCT GATGAAAAACAGGGAAGCTGCCCGGGAGTGTCGCAGGAAGAAGAAAGAATATGTCAAATGTCTTGAAAAT CGTGTGGCTGTGCTTGAAAATCAAAACAAGACCCTCATTGAGGAACTCAAGGCCCTCAAAGACCTTTATT GCCATAAAGCAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271505
Insert Size	927 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271505.1, NP_001258434.1
RefSeq Size	2612 bp
RefSeq ORF	927 bp
Locus ID	12916
UniProt ID	P27699
Gene Summary	This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters, where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes, including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (14, also known as tau 2 alpha) lacks an alternate in-frame exon and uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (14) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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