Syne4 (NM_001290565) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610012K07Rik; AI428936; KASH4; Nesp4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226925 representing NM_001290565 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGTTCCACCTCTTGGCCGTGAATTCCCGCCAGAGCCTGTGAATTGTCCCCTGGCAGCTCCTA GAGAGCTGGATGTTGTCGGAGGGACCATCTGCCCTGCACCTGAGGAAGAGACAAGCAGGCCAGAGCAGGT CCAGGCCTCCTTGGGCCTGCCTGAGCATTGCATGGGTGAACTGAAGAGCACTGAGTCTGCCACCAGCCCC TCAAGACTCCCCCTGGCCTCTTCCCATGAGCATCAAGACGGGGGCAAGCCCTGTGAGCACTCTGACTCTG GTTTGGAAGTACTAGAAGCTGAACAGGACAGTCTACATCTTTGTCTGTTGAGGTTGAACTTCCGGCTGCA GGACCTGGAACGAGGCCTTGGGTCTTGGACGCTGGCCCACAACAGGATTGTCCAGATGCAGGCCCTGCAG GCAGAGCTACGAGGGGCCGCTGAGCGCGTGGATGCATTGCTTGCATTTGGTGAGGGCCTGGCAGAGAGGA GTGAGCCCAGGGCCTGGGCATCCCTGGAGCAGGTCCTGAGGGCCCTTGGAACCCACCGAGACACCATCTT CCAACGGCTCTGGCAGCTGCAGGCCCAGTTGATCAGCTATAGCCTGGACCTGCTCTCCTTGGGGCTTGGC CACCGGAAACATTTAGCGGCTCACCATCGAAGGCGGCTCCGGAAGCCTCAGGACAGGAAGAGGCAAGTAT CCCCCAGTCTGCCTGATGCAATGCTGGAAGTGGATCGCGGGGTCCCAGCTCCTGCATCCAAGCGGCCCCT GACCCTCTTCTTTCTCCTTCTCTTCCTCCTTCTGGTGGGTGCCACGCTGTTGCTGCCCTTGTCGGGGGTC TCCTGCTGTTCTCATGCCCGGCTGGCTAGGACGCCCTACCTGGTGCTCAGTTATGTCAATGGTCTCCCTC CAATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290565
Insert Size	918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290565.1, NP_001277494.1
RefSeq Size	1105 bp
RefSeq ORF	918 bp
Locus ID	233066
Gene Summary	As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (By similarity). Behaves as a kinesin cargo, providing a functional binding site for kinesin-1 at the nuclear envelope. Hence may contribute to the establishment of secretory epithelial morphology, by promoting kinesin-dependent apical migration of the centrosome and Golgi apparatus and basal localization of the nucleus.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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