Ppt2 (NM_001302396) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610007M19Rik; AA672937
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226905 representing NM_001302396 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGCTATGGAGGCAGAGGCTTCCTTCGGCTTGGGCTTTGCTTCTCCTGCCGTTCCTGCCGCTGC TGATGCCCGCAGCCCCCGCAGCCCACCGCGGGTCCTACAAGCCCGTGATCGTGGTGCACGGGCTCTTTGA CAGTTCATACAGCTTCCGCCACCTGCTGGACTATATCAATGAGACACACACCGGGACTGTGGTGACAGTG CTTGATCTCTTCGATGGCAGAGAGAGTTTGCGGCCCCTGTGGGAACAGGTACAAGGGTTCCGAGAGGCTG TGGTCCCCATCATGGAAAAGGCCCCTGAAGGAGTGCACCTCATCTGCTACTCCCAGGGGGGCCTGGTGTG CCGTGCTTTGCTGTCTGTCATGGATAACCACAATGTGGACTCTTTCATCTCCCTCTCCTCCCCACAGATG GGCCAGTATGGAGACACGGACTATTTGAAATGGCTCTTCCCCACGTCCATGCGGTCTAACCTCTATCGGG TCTGCTATAGTCCTTGGGGCCAGGAATTTTCCATTTGCAACTACTGGCACGATCCTCACCACGATGACTT GTACCTCAATGCCAGCAGCTTTCTGGCCCTCATCAATGGGGAAAGAGACCATCCCAATGCCACTGCATGG CGGAAGAACTTCCTTCGCGTGGGCCGTCTGGTGCTGATTGGGGGTCCTGATGATGGCGTTATCACTCCCT GGCAATCTAGCTTCTTTGGTTTCTATGATGCCAATGAGACAGTTCTGGAGATGGAGGAGCAGCCGGTGTA TCTTCGAGATTCTTTTGGGTTGAAGACTCTCCTGGCCCGGGGGGCCATAGTGAGGTGTCCCATGGCTGGC ATCTCTCACACCACGTGGCACTCTAACCGTACGCTCTACGATACTTGCATTGAGCCGTGGCTCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302396
Insert Size	909 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302396.1, NP_001289325.1
RefSeq Size	1710 bp
RefSeq ORF	909 bp
Locus ID	54397
UniProt ID	O35448
Gene Summary	Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. All the variants encode the same protein.

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