St6galnac4 (NM_001276425) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	SIAT7-D; Siat7d; ST6GalNAcIV
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226900 representing NM_001276425 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCCCCGGGCCGCCTTCTGCTCCTCACACTGTGCATCTTGACATTCTCTGCTGTCTGTGTCTTCC TGTGCTGCTGGGCCTGCCTGCCGCTCTGCCTGGCAACCTGCCTGGACCGCCACCTCCCTGCGGCCCCCAG ATCCACTGTGCCAGGGCCCCTGCACTTCAGTGGCTACAGCAGCGTGCCAGACGGGAAGCCTCTTATCCGA GAACTGTGCCACAGCTGTGCGGTGGTGTCCAGCTCTGGCCAGATGCTGGGTTCAGGCCTGGGTGCCCAGA TCGATGGCGCAGAGTGCGTGCTACGCATGAACCAGGCACCCACCGTGGGCTTTGAGGAGGACGTGGGCCA GCGCAGCACTCTGCGCGTGATCTCTCACACAAGTGTGCCATTGCTTCTGCGCAACTACTCACACTATTTC CAGCATGCCCGAGACACGCTCTACGTGGTGTGGGGCCAGGGCAGGCACATGGACAGGGTGCTGGGCGGCC GCACCTACCGAACGTTGCTGCAGCTCACCAGAATGTATCCAGGCCTGCAAGTGTACACCTTCACTGAACG CATGATGGCCTACTGTGACCAGATCTTCCAAGATGAGACAGGCAAGAACCGGAGACAATCAGGCTCCTTT CTCAGCACCGGTTGGTTCACCATGATTCTGGCCCTGGAGCTGTGCGAGGAGATCGTGGTCTACGGGATGG TCAGCGATAGTTACTGCAGCGAGAAGAGTCCCCGCTCCGTGCCCTACCACTACTTTGAGAAGGGCCGGCT AGATGAGTGTCAGATGTACCGTTTGCATGAGCAGGCTCCAAGGAGCGCCCATCGCTTCATTACCGAGAAG GCTGTGTTCTCCCGCTGGGCCAAGAAGCGACCCATCGTGTTCGCCCACCCGTCCTGGAGGGCCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276425
Insert Size	909 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276425.1, NP_001263354.1
RefSeq Size	3641 bp
RefSeq ORF	909 bp
Locus ID	20448
UniProt ID	Q9R2B6
Gene Summary	Involved in the biosynthesis of ganglioside GD1A from GM1B. Transfers CMP-NeuAc with an alpha-2,6-linkage to GalNAc residue on NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc of glycoproteins and glycolipids. Prefers glycoproteins to glycolipids.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks a segment in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

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