Pqbp1 (NM_001252529) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	npw38; PQBP-1; Sfc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226852 representing NM_001252529 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACCAGTTAATTGCACCTGGGGAGAGTCGAGGAGCCTGTTGTGCTTTAAACCAAGAGCCCTTCGGT GTTGGTCTGGTCAGCTACCCACTATGCCGCTTCCTGTTGCGCTGCAGACCCGCTTGGCGAAGAGGGGCAT CCTCAAACATCTGGAGCCGGAGCCAGAGGAAGAGATTATTGCTGAAGACTACGATGATGATCCTGTTGAC TATGAGGCCACCCGGATAGAGGGTCTGCCACCGAGCTGGTACAAGGTGTTTGACCCTTCTTGCGGACTCC CTTACTATTGGAATGTGGAGACAGACCTTGTGTCGTGGCTCTCACCACATGATCCTAACTTTGTCGTTAC CAAATCCGCCAAGAAAGTCAGGAACAATAATGCAGATGCTGAGGACAAGTCGGACCGGAATCTTGAAAAG GTGGACAGAAATCATGAGAAGTCAGATCGTAGTCATGAGAAGCCAGACAGGAGCCACGAGAAGGCAGACC GAAACCACGAGAAGAATGACAGAGAACGAGAGCGCAACTACGACAAAGTGGATAGAGAGAGAGATCGGGA CAGGGAACGAGAGCGGGCATTTGACAAGGCAGACCGGGAAGAGGGCAAAGACCGACGCCACCATCGCAGA GAGGAACTGGCTCCTTACCCCAAGAACAAGAAAGCGACGAGCCGCAAAGATGAAGAATTAGACCCCATGG ACCCCAGCTCATACTCAGATGCACCCCGGGGCACATGGTCAACAGGACTCCCCAAGAGGAACGAGGCCAA GACAGGTGCTGACACCACGGCAGCTGGGCCCCTCTTCCAGCAGCGCCCTTACCCTTCCCCGGGCGCTGTG CTCCGCGCCAATGCAGAAGCCTCCCGAACCAAACAGCAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252529
Insert Size	885 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252529.1, NP_001239458.1
RefSeq Size	1119 bp
RefSeq ORF	885 bp
Locus ID	54633
UniProt ID	Q91VJ5
Gene Summary	Intrinsically disordered protein that acts as a scaffold, and which is involved in different processes, such as pre-mRNA splicing, transcription regulation, innate immunity and neuron development (By similarity). Interacts with splicing-related factors via the intrinsically disordered region and regulates alternative splicing of target pre-mRNA species (PubMed:23512658). May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner (By similarity). Can activate transcription directly or via association with the transcription machinery (By similarity). May be involved in ATXN1 mutant-induced cell death (By similarity). The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit (By similarity). Involved in the assembly of cytoplasmic stress granule, possibly by participating to the transport of neuronal RNA granules (By similarity). Also acts as an innate immune sensor of infection by retroviruses, by detecting the presence of reverse-transcribed DNA in the cytosol (By similarity). Directly binds retroviral reverse-transcribed DNA in the cytosol and interacts with CGAS, leading to activate the cGAS-STING signaling pathway, triggering type-I interferon production (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at an upstream, in-frame start codon, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1.

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