Hsd11b1 (NM_001044751) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226846 representing NM_001044751 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTTATGAAAAATTACCTCCTCCCGATCCTGGTGCTCTTCCTGGCCTACTACTACTATTCTACAA ATGAAGAGTTCAGACCAGAAATGCTCCAGGGAAAGAAAGTGATTGTCACTGGGGCCAGCAAAGGGATTGG AAGAGAAATGGCATATCATCTGTCAAAAATGGGAGCCCATGTGGTATTGACTGCCAGGTCGGAGGAAGGT CTCCAGAAGGTAGTGTCTCGCTGCCTTGAACTCGGAGCAGCCTCTGCTCACTACATTGCTGGCACTATGG AAGACATGACATTTGCGGAGCAATTTATTGTCAAGGCGGGAAAGCTCATGGGCGGACTGGACATGCTTAT TCTAAACCACATCACTCAGACCTCGCTGTCTCTCTTCCATGACGACATCCACTCTGTGCGAAGAGTCATG GAGGTCAACTTCCTCAGCTACGTGGTCATGAGCACAGCCGCCTTGCCCATGCTGAAGCAGAGCAATGGCA GCATTGCCGTCATCTCCTCCTTGGCTGGGAAAATGACCCAGCCTATGATTGCTCCCTACTCTGCAAGCAA GTTTGCTCTGGATGGGTTCTTTTCCACCATTAGAACAGAACTCTACATAACCAAGGTCAACGTGTCCATC ACTCTCTGTGTCCTTGGCCTCATAGACACAGAAACAGCTATGAAGGAAATCTCTGGGATAATTAACGCCC AAGCTTCTCCCAAGGAGGAGTGCGCCCTGGAGATCATCAAAGGCACAGCTCTACGCAAAAGCGAGGTGTA CTATGACAAATCGCCTTTGACTCCAATCCTGCTTGGGAACCCAGGAAGGAAGATCATGGAATTTTTTTCA TTACGATATTATAATAAGGACATGTTTGTAAGTAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001044751
Insert Size	879 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001044751.1, NP_001038216.1
RefSeq Size	1355 bp
RefSeq ORF	879 bp
Locus ID	15483
UniProt ID	P50172
Gene Summary	Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. An in-frame AUG is located 41 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context.

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