Tfdp1 (NM_001291768) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Dp1; Drtf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226824 representing NM_001291768 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGTGTGAGAAGGTGCAGAGGAAAGGAACCACCTCCTACAATGAGGTGGCTGACGAGCTGGTGG CAGAGTTCAGCGCTGCCGACAACCACATTCTACCAAACGAATCAGCTTATGACCAGAAGAACATCCGGCG GCGTGTCTACGATGCCTTAAATGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAA TGGATCGGCCTGCCCACCAACTCAGCTCAGGAGTGCCAGAACTTAGAGGTAGAGAGGCAGAGGAGGCTGG AGAGGATCAAACAGAAGCAGTCACAGCTCCAGGAGCTCATCCTGCAGCAAATTGCCTTCAAGAACTTGGT GCAGAGAAATCGCCAAGCTGAGCAGCAGGCCCGCAGGCCGCCTCCTCCCAACTCTGTCATCCACTTGCCC TTCATCATTGTCAACACCAGCAGGAAGACAGTCATTGACTGCAGCATCTCCAATGACAAATTTGAGTATC TGTTTAACTTTGACAACACGTTTGAGATCCACGATGACATTGAGGTGCTCAAGCGCATGGGCATGGCATG TGGGCTGGAGTCTGGCAACTGCTCTGCTGAAGACCTCAAGGTGGCCAGAAGTTTGGTACCAAAAGCTCTA GAACCATACGTGACAGAAATGGCTCAGGGATCCATTGGTGGAGTATTCGTCACGACAACAGGTTCTACAT CCAATGGCACAAGGCTTTCTGCCAGTGATTTGAGCAATGGTGCAGATGGGATGCTGGCCACGAGCTCCAA TGGGTCTCAGTACAGCGGCTCCAGGGTCGAGACCCCTGTGTCCTACGTTGGGGAGGATGATGACGACGAT GATGACTTTAATGAGAACGACGAGGAGGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291768
Insert Size	873 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291768.1, NP_001278697.1
RefSeq Size	2456 bp
RefSeq ORF	873 bp
Locus ID	21781
Gene Summary	Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, resulting in the use of a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus compared to isoform a. Variants 3, 4 and 5 encode the same isoform (b).

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