Asb13 (NM_178283) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210015B19Rik; 6430573K02Rik; Asb-13; C85285
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226743 representing NM_178283 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCGCGCCGGGGACGGCTGCTTCCTGGGAGATGTGGGTTTCTGGGTGGAGCGCACGCCCGTTC ATGAGGCGGCTCAGCGGGGTGAGAGCCTCCAGCTTCAGCAGCTGATTGACAGTGGAGCATGTGTTAACCA GGTCACTGTGGACTCGATCACACCCCTTCATGCAGCAAGTCTGCAGGGCCAGGCCCAGTGTGTGCAGCTG CTGCTAGCAGCTGGTGCCCAGGTGGACGCACGCAACATTGACGGCAGCACCCCTCTTTGTGATGCCTGTG CCTCAGGCAGCATTGAGTGTGTGAAGCTCTTGCTGTCCTATGGGGCGAAAGTCAACCCTCCTTTGTACAC AGCATCCCCGCTACACGAGGCCTGCATGAGTGGGAGTTCTGAATGCGTGAGGCTTCTTATTGATGTTGGG GCCAACCTGGAAGCCCACGATTGCCATTTTGGGACGCCTTTACATGTTGCCTGTGCCCGGGAGCATTTGG ACTGTGTCAAAGTGTTGCTCAATGCAGGGGCCAATGTGAACGCAGCCAAACTTCATGAGACTGCGCTTCA TCATGCAGCCAAGGTAAAGAACGTTGACCTCATCGAGATGCTCATAGAGTTTGGAGGCAACATCTATGCC CGGGACAACCGGGGCAAGAAGCCCTCGGACTACACGTGGAGCAGCAGCGCCCCGGCCAAGTGCTTTGAGT ACTATGAAAAGACACCTCTTAGCCTCTCCCAGCTCTGCAGGGTGAGCCTGAGGAAGGCCACCGGTGTCCG AGGACTAGAGAAAGTCGCCAAGTTGAACATCCCTCCCCGGCTCATCGACTACCTTTCCTACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_178283
Insert Size	837 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_178283.4, NP_840068.1
RefSeq Size	2410 bp
RefSeq ORF	837 bp
Locus ID	142688
UniProt ID	Q8VBX0
Gene Summary	May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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