Mtif3 (NM_001256101) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810012L14Rik; AI414549
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226734 representing NM_001256101 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTTCTTCTGATGAGGTTGATGCTACAAACTACAAAGCTTGACCATAACTTAATTGGAAGATGTT TGCAAAGACACGCTGTGAAGCCAGATCCAGCACAGCTGTCCCTGTCTGCGTCCACCCCAAAGCTGTTGTA CCTGACTTCTGCAAAAGGTTTTAGTACTGCTGGAGACCCACAGGGTGAGAGGAAACAGAAAAGAAGGGAT GCTTTCTCTAACACCGGCCGGAAGATTAGTGAGCGGATTATCCGCGTCCTGGATGAGAAGGGCATGGACC TGGGGATGATGCATCGAGCAGATGTGATCAGACTCATGAACAAGCAGGACCTGAGGCTGGTGCAGAGGAA CACCAGCTCAGAGCCACCGGAGTACCAGCTTATGACCGGAGAGCAGATCCACCAGGAGCGGCTAAAGCTC CGGGAACAGGAAAAGGCCAAACCCAAAACTGGACCAACTATGACGAAAGAGCTCGTTTTCTCTTCAAATA TTGGACAGCACGATTTAGACACCAAGAGTAAACAGATCCAGCAGTGGATAGAGAAGAAATACCACGTTCA AGTCACGATAAAGAGGAGGAAAGATGCGGAGCAGTCAGAGGAGGAAACGGAGGAGATATTTAACCAGATT CTCCAGACTATGCCTGACATAGCAACCTTCTCAAGCAGACCAAAAGCCATCAGAGGAGGGACTGCTTCCA TGTGTGTTTTTCGTCACTTGAGCAAGAAAGAAGAAAAGGCGTACAGAGAATCCCAGGAGAGCCAGAGAAG AGACACTTTGAGCAAAGACGACGACGGAAACAGCAAAGAGTCGGATGTTGTGTGTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256101
Insert Size	831 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256101.1, NP_001243030.1
RefSeq Size	5128 bp
RefSeq ORF	831 bp
Locus ID	76366
UniProt ID	Q9CZD5
Gene Summary	IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All five variants encode the same protein.

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