Fbxl12 (NM_001286530) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110048D16Rik; Fbl12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226717 representing NM_001286530 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCGAAAGTCATGTGGCACCTCCTGCGCCGGTACATGGCGTCCCGGCTCTACTCGTTGCGCATGG GCGGCTACCTGTTTTCTGGCTCTCAGGCCCCACAGCTGTCCCCCGCCTTGATGAGGGCCCTGGGTCAGAA ATGCCCCAATCTGAAGCGCCTGTGCCTGCACGTGGCTGACCTGAGCATGGTGCCTATCACCAGCCTTCCT AGCACACTGAGGACCCTGGAGCTGCACAGCTGCGAAATCTCCATGATCTGGTTGCAGAAAGAGCAGGACC CCACGGTGCTGCCTCTGCTGGAATGCATCGTGCTGGACCGAGTGCCGGCCTTTCGCGATGAGCATCTGCA GGGCCTTACCCGATTCCGAGCCCTGCGCTCGCTGGTGCTGGGCGGCACCTACCGGGTCACTGAGACCGGG CTAGATGCCAGCCTGCAGGAGCTCAGCTACCTGCAAAGGCTTGAGGTGCTGGGCTGCACCCTGTCAGCTG ACAGCACGCTGCTGGCCATCAGCCGCCACCTTCGAGATGTGCGCAAGATTCGGCTGACCGTTGGGGGCCT CTCAGCCCAGGGCCTGGTCTTCCTGGAGGGAATGCCTGTCCTGGAGAGTTTGTGCTTCCAGGGTCCCCTT ATCACCCCAGACATGCCCACACCCACTCAGATTGTGTCCTCCTGCCTCACCATGCCGAAGCTCAGAGTGC TTGAGGTGCAAGGGCTGGGCTGGGAGGGTCAGGAAGCAGAGAAAATCCTGTGCAAGGGCCTGCCCCACTG CATAGTTATTGTTAGGGCTTGTCCCAAAGAATCCATGGATTGGTGGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286530
Insert Size	822 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286530.1, NP_001273459.1
RefSeq Size	1822 bp
RefSeq ORF	822 bp
Locus ID	30843
UniProt ID	Q9EPX5
Gene Summary	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform b, which has a shorter N-terminus, compared to isoform a. Variants 2 and 4 encode the same protein.

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