Srsf5 (NM_001079694) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Sfrs5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226701 representing NM_001079694 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCTGTCGAGTGTTCATTGGGAGACTAAATCCAGCAGCGAGGGAGAAAGATGTGGAAAGATTCT TCAAGGGTTACGGACGGATCCGAGATATTGACTTGAAAAGAGGTTTTGGTTTTGTGGAATTTGAGGACCC AAGGGATGCAGATGATGCTGTTTATGAACTTGATGGGAAGGAACTTTGCAGTGAAAGGGTGACGATTGAA CATGCCCGGGCTCGGTCTCGAGGTGGAAGAGGTAGAGGACGATACTCCGACCGTTTTAGCAGTCGCAGAC CTCGAAATGATAGACGAAATGCTCCACCTGTAAGAACAGAAAATCGACTTATAGTTGAGAATTTATCCTC AAGAGTCAGCTGGCAGGATCTCAAAGATTTCATGAGACAAGCTGGGGAAGTAACCTTTGCGGATGCACAT CGACCTAAACTAAATGAAGGGGTAGTTGAGTTTGCCTCTTATGGTGACTTAAAGAATGCTATTGAGAAAC TTTCTGGAAAGGAAATTAACGGGAGAAAAATCAAATTAATTGAAGGCAGCAAAAGGCACAGGTCAAGAAG CAGGTCACGATCTCGGACCAGAAGTTCCTCTAGGTCCCGTAGCCGATCCCGTTCACGAAGGAGCAAGTCT TACAGCCGATCAAGGAGCAGGAGCCGGAGCCGGAGCAAGTCCCGTTCTGGTAGTAGGTCTCCTGTGCCTG AGAAGAGCCAGAAACGTGGTTCTTCGAGTAGATCTAAATCTCCAGCATCTGTGGATCGCCAGAGGTCCCG ATCCCGGTCCAGGTCCAGATCAGTTGACAGTGGCAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001079694
Insert Size	810 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001079694.1, NP_001073162.1
RefSeq Size	1536 bp
RefSeq ORF	810 bp
Locus ID	20384
UniProt ID	O35326
Gene Summary	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-3 encode the same isoform (1).

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