Gzmm (NM_001302485) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Lmet; Lmet1; MMET-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226662 representing NM_001302485 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTCTGCTGGTCCCTGCTGCTACTGCTGGCCCTGAAAACACTGTGGGCAGGCAACAGATTTGAGA CCCAGATCATTGGGGGTCGAGAGGCAGTCCCGCACTCCCGCCCATACATGGCCTCTCTACAGAAAGCCAA GTCCCATGTGTGTGGGGGAGTCCTTGTGCATCGGAAGTGGGTATTGACAGCTGCCCACTGCCTGTCTGAG CCGCTACAGAACCTGAAGCTGGTGCTTGGCCTGCACAACCTCCATGATCTCCAAGATCCTGGCCTCACCT TCTACATCCGGGAAGCCATTAAACACCCTGGCTACAACCACAAATATGAGAACGACCTGGCACTGCTTAA GCTAGATAGACGAGTGCAGCCCAGCAAGAATGTCAAACCACTAGCTCTGCCAAGAAAGCCCCGATCCAAG CCGGCAGAAGGTACCTGGTGCAGCACAGCTGGCTGGGGAATGACCCACCAGGGTGGGCCCCGGGCCAGGG CCCTGCAGGAGTTGGATCTGCGTGTGCTGGATACCCAAATGTGTAACAACAGCCGCTTCTGGAACGGTGT CCTCATAGACAGCATGCTATGCTTAAAGGCTGGGAGCAAGAGCCAAGCCCCCTGCAAGGGTGACTCTGGA GGGCCCCTGGTGTGTGGCAAAGGCCAGGTGGATGGGATCCTGTCTTTCAGCTCCAAAACCTGCACAGACA TCTTCAAGCCACCTGTGGCCACTGCTGTAGCCCCCTACAGCTCCTGGATCAGGAAGGTCATTGGTCGCTG GTCACCCCAATCTTTGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302485
Insert Size	792 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302485.1, NP_001289414.1
RefSeq Size	1301 bp
RefSeq ORF	792 bp
Locus ID	16904
Gene Summary	The protein encoded by this gene is a member of a family of cytotoxic lymphocyte serine proteases called granzymes, which are expressed by cytotoxic T lymphocytes and natural killer cells. This protein belongs to a subfamily of granzymes that cleave after methionine residues. Natural killer cell development, homeostasis and cytotoxicity are normal in mice deficient for this gene, but they demonstrate increased susceptibility to murine cytomegalovirus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) utilizes an alternate in-frame splice site in the 5' coding region. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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