Eif4e1b (NM_001286178) Mouse Untagged Clone

CAT#: MC226573

Eif4e1b (untagged) - Mouse eukaryotic translation initiation factor 4E family member 1B (Eif4e1b), transcript variant 3



  "NM_001286178" in other vectors (1)

CNY 2,570.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Eif4eloo; Gm273
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC226573 representing NM_001286178
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTTCTGAGATGAACAAAGTTGAGGGTGGAGGGCATAAGGAGGAGGTGGTGGTGAAAGAAAAGG
AGGTAGTGAAAGAAAAGCCATCAGAAGCAACTGCGGAGGGAGTCCAAGCAGGAGAAGCCAAAGACCTTCC
TGGCTCCCTTAAGACTCAGAGGAGGAAGGCCCACAGAGAACATCCACCAGAGGTCCTGAGCAAGCTACAC
CCCTTGCAGTACAGGTGGGTTCTGTGGTTCTTCAAGAATGACCGAAGCCGGGCCTGGCAGGACAACCTGC
AGCTAGTCACCAAGTTTAACACTGTGGAGGACTTCTGGGCGGTGTACAGCCATATCAAGCTGGCCAGCAA
GCTCTCTTCCGGCTGTGACTATGCCCTGTTCAAGGAAGGCATCCTGCCCATGTGGGAAGACAACAGAAAC
AAGCAGGGTGGCCGCTGGCTGCTCAGCATTGACAAACAACTGCGCCACTTCGAACTGGACCGTCTGTGGC
TGGAGACGTTGCTGTGTCTGGTTGGGAATTGTTTTGAGGAATACAGCAGGGAAGTGTGCGGTGCTGTCGT
GAACATCCGCACGAAGAGGGACAAGATTGCCCTGTGGACGAGTGAAGCAGAGGACAAAGCCGGAGTGATG
CAGATCGGGCAAATATACAAAGAGCGCCTGGGCATCTCTACGAAGACCATCATTGGGTACCAGGCCCATG
CAGACACTGCTGCCAAGAGCAACAACTTAGCAAACAAGTTTGTGGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001286178
Insert Size 750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286178.1, NP_001273107.1
RefSeq Size 1848 bp
RefSeq ORF 750 bp
Locus ID 218268
UniProt ID Q3UTA9
Gene Summary Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses an alternate splice site in the 5'-terminal exon, and initiates translation from an alternate start codon, compared to variant 1. The encoded isoform (3) has a longer N-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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