Eif4e1b (NM_001286178) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Eif4eloo; Gm273
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226573 representing NM_001286178 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTTCTGAGATGAACAAAGTTGAGGGTGGAGGGCATAAGGAGGAGGTGGTGGTGAAAGAAAAGG AGGTAGTGAAAGAAAAGCCATCAGAAGCAACTGCGGAGGGAGTCCAAGCAGGAGAAGCCAAAGACCTTCC TGGCTCCCTTAAGACTCAGAGGAGGAAGGCCCACAGAGAACATCCACCAGAGGTCCTGAGCAAGCTACAC CCCTTGCAGTACAGGTGGGTTCTGTGGTTCTTCAAGAATGACCGAAGCCGGGCCTGGCAGGACAACCTGC AGCTAGTCACCAAGTTTAACACTGTGGAGGACTTCTGGGCGGTGTACAGCCATATCAAGCTGGCCAGCAA GCTCTCTTCCGGCTGTGACTATGCCCTGTTCAAGGAAGGCATCCTGCCCATGTGGGAAGACAACAGAAAC AAGCAGGGTGGCCGCTGGCTGCTCAGCATTGACAAACAACTGCGCCACTTCGAACTGGACCGTCTGTGGC TGGAGACGTTGCTGTGTCTGGTTGGGAATTGTTTTGAGGAATACAGCAGGGAAGTGTGCGGTGCTGTCGT GAACATCCGCACGAAGAGGGACAAGATTGCCCTGTGGACGAGTGAAGCAGAGGACAAAGCCGGAGTGATG CAGATCGGGCAAATATACAAAGAGCGCCTGGGCATCTCTACGAAGACCATCATTGGGTACCAGGCCCATG CAGACACTGCTGCCAAGAGCAACAACTTAGCAAACAAGTTTGTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286178
Insert Size	750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286178.1, NP_001273107.1
RefSeq Size	1848 bp
RefSeq ORF	750 bp
Locus ID	218268
UniProt ID	Q3UTA9
Gene Summary	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the 5'-terminal exon, and initiates translation from an alternate start codon, compared to variant 1. The encoded isoform (3) has a longer N-terminus, compared to isoform 1.

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