Vti1a (NM_001293685) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110014F16Rik; 1110018K19Rik; 4921537J05Rik; MVti1; MVti1a; Vti1; Vti1-rp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226393 representing NM_001293685 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCGACTTCGAAGGGTACGAGCAGGACTTCGCAGTGCTCACTGCTGAGATCACCAGCAAGATTG CAAGGGTCCCTCGGCTCCCTCCCGATGAGAAGAAGCAAATGGTTGCCAATGTGGAGAAACAGCTTGAAGA GGCCAGAGAACTGCTTGAACAGATGGATTTGGAAGTTCGAGAAATTCCACCCCAAAGTCGAGGAATGTAT AGCAACAGGATGAGAAGCTACAAGCAAGAAATGGGGAAACTGGAAACGGATTTTAAAAGGTCACGGATCG CCTACAGTGACGAAGTACGGAATGAGCTCCTAGGGGATGCTGGGAATTCCTCCGAGAACCAGTTGATAAA ATTACGTGAAGAGAGGGCACATCTGCTGGATAACACGGAGAGGCTGGAAAGGTCGTCTCGGAGACTAGAG GCTGGGTACCAGATAGCAGTGGAGACTGAGCAAATCGGCCAAGAGATGTTGGAAAACCTGAGTCATGACA GAGAAAAGATACAGCGGGCACGTGACCGACTCCGGGACGCAGATGCAAACCTGGGGAAGAGCTCTCGGAT TCTGACGGGGATGCTGCGAAGAATCATCCAGAACCGTATCCTGCTCGTCATCTTGGGGATCATCGTGGTC ATCGCCATCCTGACGGCCATCGCGTTTTTCGTCAAAGGACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293685
Insert Size	675 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293685.1, NP_001280614.1
RefSeq Size	4620 bp
RefSeq ORF	675 bp
Locus ID	53611
Gene Summary	V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence.[UniProtKB/Swiss-Prot Function]

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