Scn3b (NM_001083917) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110001K16Rik; 4833414B02Rik; Scnb3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226342 representing NM_001083917 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCCTTCAACAGATTGCTTCCCCTAGCTTCTCTAGTGCTCATCTACTGGGTCAGAGTCTGCTTCC CTGTGTGTGTAGAAGTACCCTCGGAGACAGAAGCCGTGCAGGGCAATTCCATGAAGCTGAGATGCATCTC CTGCATGAAGAGGGAGGAGGTGGAGGCCACCACTGTAGTGGAGTGGTTCTACAGGCCTGAGGGCGGTAAA GATTTCCTTATATATGAGTATCGAAATGGCCACCAGGAGGTGGAGAGCCCCTTCCAAGGTCGTCTGCAGT GGAATGGGAGCAAAGACCTGCAGGACGTATCCATCACTGTTCTCAATGTCACTCTGAATGACTCTGGCCT CTACACATGTAATGTGTCCAGGGAGTTTGAGTTCGAAGCACACCGGCCCTTTGTGAAGACCACAAGACTA ATACCCCTGCGAGTCACTGAAGAGGCGGGAGAAGACTTCACCTCCGTGGTCTCGGAAATCATGATGTACA TCCTCCTGGTCTTCCTCACCTTGTGGCTGTTTATTGAGATGATCTATTGCTACAGAAAGGTCTCTAAGGC CGAAGAGGCAGCTCAGGAAAATGCGTCTGACTACCTTGCTATCCCTTCAGAGAACAAGGAGAACTCTGTG GTACCCGTGGAGGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001083917
Insert Size	648 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001083917.1, NP_001077386.1
RefSeq Size	4177 bp
RefSeq ORF	648 bp
Locus ID	235281
UniProt ID	Q8BHK2
Gene Summary	Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with NFASC may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 3' UTR, compared to variant 2. Variants 2, 3 and 5 encode the same protein (isoform a).

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